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Method for extracting collagen from giant salamander

A collagen and giant salamander technology, applied in the biological field, can solve the problems of affecting application value, waste of resources, low purification rate and purity of collagen, and achieve the effect of improving market application value, extraction rate and purity

Active Publication Date: 2017-09-26
GUIZHOU UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, the molting of the artificially cultured giant salamander is treated, enzymatically hydrolyzed, filtered, concentrated, spray-dried, etc., and finally collagen is obtained, and its molecular weight is below 3000 Daltons. Due to the diversity and irrationality of extraction methods, the purification rate and purity of collagen in the later stage are low, which seriously affects its application value and wastes resources. Therefore, how to improve on the basis of traditional giant salamander extraction methods It is particularly important to improve the extraction rate and purity of collagen

Method used

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  • Method for extracting collagen from giant salamander
  • Method for extracting collagen from giant salamander

Examples

Experimental program
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Embodiment 1

[0029] A method for extracting collagen from giant salamanders, comprising the following steps:

[0030] (1) Sterilization treatment: After the giant salamander raw material is pretreated, add 0.8 times the amount of sterilizing solution of the giant salamander raw material quality, adjust the temperature of the magnetic stirrer to 36 ° C, stir for 40 minutes, filter, and wash the filter residue with deionized water for 3 to 5 minutes. Second-rate;

[0031] The giant salamander raw material is mixed with giant salamander skin, giant salamander muscle and giant salamander bone in a mass ratio of 1:0.2:0.4;

[0032] The pretreatment is to remove impurities from the giant salamander raw material, dry and pulverize it, mix it with n-hexane and sterolase at a mass ratio of 1.8:0.3:0.1, add deionized water, and blend into a solution with a content of 50%. Heat to 38°C, stir for 100 minutes, and filter to get the filter residue;

[0033] The sterilizing solution is to mix dandelion...

Embodiment 2

[0045] A method for extracting collagen from giant salamanders, comprising the following steps:

[0046] (1) Sterilization treatment: After the giant salamander raw material is pretreated, add 1.2 times the sterilizing solution of the giant salamander raw material quality, adjust the temperature of the magnetic stirrer to 38°C, stir for 50 minutes, filter, and wash the filter residue with deionized water for 3 to 5 minutes. Second-rate;

[0047] The giant salamander raw material is mixed with giant salamander skin, giant salamander muscle and giant salamander bone in a mass ratio of 1:0.3:0.5;

[0048] The pretreatment is to remove impurities from the giant salamander raw material, dry and pulverize it, mix it with n-hexane and sterolase at a mass ratio of 2.2:0.5:0.2, add deionized water, and blend it into a solution with a content of 60%. Heat to 42°C, stir for 120 minutes, and filter to get the filter residue;

[0049] The sterilizing solution is to mix dandelion and worm...

Embodiment 3

[0061] A method for extracting collagen from giant salamanders, comprising the following steps:

[0062] (1) Sterilization treatment: After the giant salamander raw material is pretreated, add a sterilizing solution of 1 times the mass of the giant salamander raw material, adjust the temperature of the magnetic stirrer to 37°C, stir for 45 minutes, filter, and wash the filter residue with deionized water for 3 to 5 minutes. Second-rate;

[0063] The giant salamander raw material is mixed with giant salamander skin, giant salamander muscle and giant salamander bone in a mass ratio of 1:0.25:0.45;

[0064] The pretreatment is to remove impurities from the giant salamander raw material, dry and pulverize it, mix it with n-hexane and sterolase at a mass ratio of 2:0.4:0.15, add deionized water, and blend it into a solution with a content of 55%. Heat to 40°C, stir for 110 minutes, and filter to get the filter residue;

[0065] The sterilizing solution is to mix dandelion and wor...

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Abstract

The invention relates to the field of biology, and in particular to a method for extracting collagen from giant salamander. The method comprises the following steps: sterilization treatment, acid leaching, water washing, alkaline leaching, secondary water washing, salt leaching, enzymolysis and purifying. According to the method disclosed by the invention, the collagen is extracted by utilizing a mixture of the skin, the muscle and the bones of the giant salamander as a raw material, and the resource of the giant salamander is fully utilized; through the operation steps of sterilization treatment, acid leaching, water washing, alkaline leaching, secondary water washing, salt leaching, enzymolysis and purifying, the extracting rate and the purity of giant salamander collagen are effectively increased, determination shows that the extracting rate is up to 75.2 percent and the purity is up to 97.8 percent, the demands on the collagen and the pursuit to quality of a current market are effectively met, and the market application value of the collagen is increased.

Description

technical field [0001] The invention relates to the biological field, in particular to a method for extracting collagen from giant salamanders. Background technique [0002] Giant salamander, also known as giant salamander, belongs to Amphibian Caurus order and is known as soft gold. It is rich in protein and trace elements, as well as a small amount of fat. Among them, 8 kinds of amino acids are essential amino acids for human body. They not only have high nutritional value, but also can Prevent many diseases, improve human immunity and cell metabolism. At the same time, giant salamander has high application value in food and medicine, such as: beauty and beauty, prevention of cardiovascular diseases, anti-aging, anti-cancer, etc. It is a very good health food. In recent years, with people's understanding of the giant salamander, there are more and more researches on the giant salamander at home and abroad. In order to better utilize the nutritional value and medicinal valu...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/36C07K1/30C07K1/34
CPCC07K14/78C12P21/06
Inventor 欧阳力剑
Owner GUIZHOU UNIV OF ENG SCI
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