Ultrasonic-assisted corn protein extraction method
A corn gluten and corn technology, applied in the field of corn gluten extraction technology, can solve the problems of high protein yield and purity, low extraction rate and purity, low solvent consumption, etc., so as to shorten the extraction time, improve the extraction rate and purity, The effect of reducing energy consumption
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[0025]According to a preferred embodiment of the present invention, the corn fermentation mash contains 3-4% by weight of crude protein.
[0026]According to the present invention, in step 1, the first solid-liquid separation method can be various conventional methods for separating the solid phase and the liquid phase in the mixed liquid, for example, filtration, centrifugation, and the like. According to a preferred embodiment of the present invention, the first solid-liquid separation is centrifugation. Wherein, the conditions of the centrifugation can be selected in a wide range. Preferably, the conditions of the centrifugation are: the rotation speed is 3000-10000rpm, for example, it can be 3000rpm, 3500rpm, 4000rpm, 4500rpm, 5000rpm, 6000rpm, 7000rpm, 8000rpm, 9000rpm, 10000rpm, preferably 3000-8000rpm, more preferably 3500-4500rpm; time is 5-30min, for example, 5min, 10min, 15min, 20min, 25min, 30min, preferably 15-25min.
[0027]According to the present invention, in step 1, the m...
Example Embodiment
[0058]Example 1
[0059]This example is used to illustrate the method for extracting corn protein from corn fermented mash provided by the present invention
[0060](1) Centrifuge the fermented mash at 4000rpm for 20min, collect 10g of distiller’s grain solids, use ethanol as the extract, prepare the extract so that the ethanol content is 30% by volume, adjust the pH to 9 with sodium hydroxide, and obtain the liquid in the system The ratio is 1:10, add system 0.2% (g / ml) sodium sulfite, stir and mix well.
[0061](2) Ultrasound the above suspension, modulate 60kHz, 50°C for 120min;
[0062](3) Separate the solid-liquid suspension after the ultrasonic treatment, collect the extract, and distill under reduced pressure to remove part of the organic solvent, but no protein is precipitated.
[0063](4) Adjust the pH of the extract after distillation to 4.7, centrifuge to collect the precipitated zein, adjust the pH to 6.4 again, centrifuge to collect the precipitated corn gluten, and then combine the o...
Example Embodiment
[0065]Example 2
[0066]This example is used to illustrate the method for extracting corn protein from corn fermented mash provided by the present invention
[0067](1) Centrifuge the fermented mash, 4000rpm, 20min, collect 10g of distiller's grain solids, use ethanol as the extract, prepare the extract so that the ethanol content is 50% by volume, adjust the pH to 9.5 with sodium hydroxide, and obtain the liquid in the system The ratio is 1:15, add 0.1% (g / ml) sodium sulfite to the system, stir and mix well.
[0068](2) Ultrasound the above suspension, modulate 65kHz, 60°C for 130min.
[0069](3) Separate the solid-liquid suspension after the ultrasonic treatment, collect the extract, and distill under reduced pressure to remove part of the organic solvent, but no protein is precipitated.
[0070](4) Adjust the pH of the extract after distillation to 4.7, centrifuge to collect the precipitated zein, adjust the pH to 6.4 again, centrifuge to collect the precipitated corn gluten, and then combine t...
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