Ultrasonic-assisted corn protein extraction method

A corn gluten and corn technology, applied in the field of corn gluten extraction technology, can solve the problems of high protein yield and purity, low extraction rate and purity, low solvent consumption, etc., so as to shorten the extraction time, improve the extraction rate and purity, The effect of reducing energy consumption

A corn gluten and corn technology, applied in the field of corn gluten extraction technology, can solve the problems of high protein yield and purity, low extraction rate and purity, low solvent consumption, etc., so as to shorten the extraction time, improve the extraction rate and purity, The effect of reducing energy consumption

CN112209998AActive Publication Date: 2021-01-12GUOTOU BIO TECH INVESTMENT CO LTD +2
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  • Ultrasonic-assisted corn protein extraction method
  • Ultrasonic-assisted corn protein extraction method
  • Ultrasonic-assisted corn protein extraction method

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0025] According to a preferred embodiment of the present invention, the corn fermented mash contains 3-4% by weight of crude protein.

[0026] According to the present invention, in step 1, the first solid-liquid separation method can be various conventional methods for separating the solid phase and the liquid phase in the mixed liquid, for example, it can be filtration, centrifugation and the like. According to a preferred embodiment of the present invention, the first solid-liquid separation is centrifugation. Wherein, the condition of the centrifugation can be selected in a wide range, preferably, the centrifugation condition is: the rotating speed is 3000-10000rpm, for example, it can be 3000rpm, 3500rpm, 4000rpm, 4500rpm, 5000rpm, 6000rpm, 7000rpm, 8000rpm, 9000rpm, 10000rpm, preferably 3000-8000rpm, more preferably 3500-4500rpm; time is 5-30min, for example, 5min, 10min, 15min, 20min, 25min, 30min, preferably 15-25min.

[0027] According to the present invention, in s...

Embodiment 1

[0059] This embodiment is used to illustrate the method for extracting corn protein from corn fermented mash provided by the present invention

[0060] (1) Centrifuge the fermented mash, 4000rpm, 20min, collect 10g of distiller's grain solids, use ethanol as the extract, prepare the extract so that the ethanol content is 30% by volume, adjust the pH to 9 with sodium hydroxide, and obtain the feed liquid in the system The ratio is 1:10, add 0.2% (g / ml) sodium sulfite to the system, stir and mix well.

[0061] (2) Ultrasonic the above suspension, modulated at 60kHz, and ultrasonicated at 50°C for 120min;

[0062] (3) Separating the solid-liquid of the above-mentioned ultrasonically treated suspension, collecting the extract, and distilling under reduced pressure to remove part of the organic solvent, but no protein was precipitated.

[0063] (4) Adjust the pH of the above-mentioned distilled extract to 4.7, centrifuge to collect the precipitated zein, adjust the pH to 6.4 again...

Embodiment 2

[0066] This embodiment is used to illustrate the method for extracting corn protein from corn fermented mash provided by the present invention

[0067] (1) Centrifuge the fermented mash, 4000rpm, 20min, collect 10g of distiller's grain solids, use ethanol as the extract, prepare the extract so that the ethanol content is 50% by volume, adjust the pH to 9.5 with sodium hydroxide, and obtain the feed liquid in the system The ratio is 1:15, add 0.1% (g / ml) sodium sulfite to the system, stir and mix well.

[0068] (2) Sonicate the above suspension at 65kHz, and sonicate at 60°C for 130min.

[0069] (3) Separating the solid-liquid of the above-mentioned ultrasonically treated suspension, collecting the extract, and distilling under reduced pressure to remove part of the organic solvent, but no protein was precipitated.

[0070] (4) Adjust the pH of the above-mentioned distilled extract to 4.7, centrifuge to collect the precipitated zein, adjust the pH to 6.4 again, centrifuge to c...

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Abstract

The invention relates to a corn protein extraction process, in particular to an ultrasonic-assisted corn protein extraction method. The method comprises the following steps: step 1, carrying out firstsolid-liquid separation on corn fermented mash, collecting vinasse solids, adding an extracting solution containing an organic solvent to enable the pH range of the system to be 7-11, and then addinga reducing agent; step 2, carrying out ultrasonic treatment on the system; step 3, after ultrasonic treatment, carrying out second solid-liquid separation to obtain an extracting solution rich in corn protein, and carrying out reduced pressure distillation to recover part of the organic solvent to obtain a concentrated solution; and step 4, adjusting the pH value of the concentrated solution to aprotein isoelectric point, collecting precipitated target protein, washing the target protein to be neutral, and performing drying to obtain a corn protein finished product. The method can be used for extracting alcohol-soluble protein and glutelin in the corn fermented mash at the same time, and is low in solvent consumption, short in time and high in protein yield and purity.

Description

technical field [0001] The invention relates to a process for extracting corn protein, in particular to a method for ultrasonically assisted extraction of corn protein. Background technique [0002] Corn is one of the three most widely distributed food crops in the world. The planting area is second only to wheat and rice. my country's average annual corn production and consumption ranks second in the world, second only to the United States. With the development of the corn alcohol industry, a large amount of by-product corn fermented mash will be produced, but it is easy to deteriorate and not easy to store for a long time. If it is not treated effectively, it will not only pollute the environment, but also cause a great waste of resources. [0003] At present, corn fermented mash is mostly used in the preparation of animal feed, for example, as a by-product of fuel ethanol, distiller's dried grains and solubles (DDGS), which use corn as raw material, are obtained after liq...

Claims

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Application Information

Patent Timeline
12 Jan 2021
Publication
CN112209998A
IPC
C07K14/425; C07K14/415; C07K1/30; C07K1/14
CPC
C07K14/425; C07K14/415; Y02P60/87
Inventors
石飞虹; 邓立康