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Method for extracting active protein from rice bran

A technology of active protein and rice bran, which is applied in the field of natural product extraction, can solve the problem of low purity of active protein, achieve high purity, increase the content of active protein, and promote the effect of cell wall rupture

Inactive Publication Date: 2017-06-06
嵊州市派特普科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventive method can successively and efficiently extract phytic acid, fat and protein from full-fat rice bran, realize the high-value full utilization of full-fat rice bran, increase its added value, and avoid waste of resources, but the invention extracts rice bran active protein The purity is not high, and there is still room for improvement in the extraction rate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for extracting active protein from rice bran, the method comprises the following steps:

[0024] 1) Pretreatment: add isopropanol to the rice bran, stir, add n-hexane to extract, adjust the slurry, pour it into a colloid mill, centrifuge, take the rice bran meal for vacuum precipitation, and obtain the defatted rice bran meal;

[0025] 2) Enzymolysis and extraction: add distilled water to the rice bran meal, microwave extraction, adjust the pH, add 1-2% by weight of rice bran compound enzyme, stir, inactivate the enzyme, and centrifuge to get the supernatant;

[0026] 3) Separation and purification: ultrafiltration, gel chromatography, and freeze-drying to obtain active protein; the present invention performs degreasing, microwave extraction, enzymatic hydrolysis, separation and purification on rice bran to increase the active protein content of the extract and obtain the purity of the active protein high.

[0027] The distilled water in step 2) contains 0.1-0...

Embodiment 2

[0036] A method for extracting active protein from rice bran, the method comprises the following preferred steps:

[0037] 1) Pretreatment: add isopropanol to rice bran, the volume ratio of rice bran to isopropanol is preferably 1:3, stir, add n-hexane for extraction, the volume ratio of rice bran to n-hexane is preferably 1:3, and mix , poured into the colloid mill for 9 minutes, and centrifuged at 3500r / min for 8 minutes to obtain rice bran meal and mixed oil, take rice bran meal, the vacuum degree of vacuum precipitation is preferably 0.1MPa, and the temperature is preferably 52°C to obtain defatted rice bran meal;

[0038]2) Enzymolysis and extraction: add distilled water 38 times the weight of the preferred rice bran, microwave extraction, the microwave power is preferably 380w, microwave extraction at 52°C for 3 times, preferably 40min each time, adjust the pH to 6.1, add the preferred rice bran 1.2% by weight compound enzyme, stirred for 55 minutes, the enzymolysis temp...

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Abstract

The invention provides a method for extracting active protein from rice bran. The method comprises the steps: 1) pretreatment: adding isopropanol into the rice bran, stirring, adding normal hexane to extract, mixing size, pouring into a colloid mill, centrifuging, taking rice bran meal and desolventizing the rice bran meal in vacuum to obtain degreased rice bran meal; 2) enzymolysis and extraction: adding distilled water into the rice bran meal, microwave extracting, adjusting pH, adding complex enzyme of 1 to 2% weight part of the rice bran meal, stirring, denaturing enzyme, centrifuging and taking supernatant; 3) separation and purification: performing ultrafiltration and gel chromatography, freezing and drying to obtain the active protein. The method disclosed by the invention can improve the content of the active protein in an obtained extract by degreasing, microwave extracting, performing enzymolysis on, separating and purifying the rice bran meal, and the obtained active protein has high purity.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting active protein from rice bran. Background technique [0002] Rice bran is one of the most important by-products in rice processing. Rice bran is not only rich in lipids (16-22%) and crude protein (12-16%), but also contains a variety of natural active substances, such as phytic acid, oryzanol, γ-aminobutyric acid, lipopolysaccharide, tocopherol, etc. At present, the annual output of rice bran in my country has reached 18 million tons, but the utilization rate is only 10%, most of which are used as low-value feed, causing great waste. [0003] Rice bran protein is a plant protein with high nutritional value, of which soluble protein accounts for about 70%. The essential amino acid composition is close to the human body's need mode, and the content of lysine and methionine is higher than that of rice and other grains, which ca...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16C07K1/14
CPCC07K1/145C07K1/16C07K1/34C07K1/36C12P21/06
Inventor 施春华张美芬
Owner 嵊州市派特普科技开发有限公司
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