mTAT-hEGF-kCD47 fusion protein, construction method and application

Abstract

The invention discloses mTAT-hEGF-kCD47 fusion protein. mTAT, kCD47 and hEGF are subjected to fusion expression, a complete genome sequence of mTAT-hEGF-kCD47 is designed, after artificial synthesis is conducted, the complete genome sequence of the mTAT-hEGF-kCD47 is connected with the interior of a pTrcHisB expression vector, an mTAT-hEGF-kCD47 recombinant expression plasmid vector is constructedsuccessfully, through expression and purification, the mTAT-hEGF-kCD47 fusion protein with biological activity is obtained, and the mTAT-hEGF-kCD47 fusion protein has the advantages of being low in cost, high in activity and long in action time. According to the mTAT-hEGF-kCD47 fusion protein, not only are original penetrating power of TAT and the action of CD47 of inhibiting nonspecific immune response reserved, but also the activity of the foreign protein hEGF is almost not affected, so that the mTAT-hEGF-kCD47 fusion protein has wide prospects in preparing medicines and make-ups for promoting epidermis proliferation.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to an mTAT-hEGF-kCD47 fusion protein, and also relates to a construction method and application of the mTAT-hEGF-kCD47 fusion protein. Background technique [0002] Epidermal growth factor (EGF) has various functions such as promoting the proliferation of epidermal cells. Human epidermal growth factor (hEGF) is a single-chain polypeptide composed of 53 amino acid residues, its molecular weight is 6kDa, and its isoelectric point pI is 4.2. The hEGF molecule has high stability and can withstand the effects of acid, alkali, heat and pepsin and trypsin. A large number of studies have confirmed that hEGF has the ability to stimulate the proliferation and differentiation of epidermal cells. It can accelerate the process of new cells replacing aging cells by promoting the synthesis of DNA in epidermal cells, increase the uptake of other endogenous growth factors by ...

AI Technical Summary

Problems solved by technology

[0008] The first object of the present invention is to provide a mTAT-hEGF-kCD47 fusion protein, which solves the problem that macromolecular proteins cannot actively penetrate the epidermis and are easily cleared by the immune system in the skin

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