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Method for reinforcing soil body by combination of organic matter and plant urease

A technology of plant urease and organic matter, which is applied in geotechnical engineering reinforcement and foundation treatment, can solve the problems of EICP reinforcement soil overall effect deviation, lack of nucleation sites, and influence on calcium carbonate deposition, so as to improve mineral morphology and structure, Resource rich, effects that increase strength and effect

Inactive Publication Date: 2020-10-27
HENAN UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

However, since plant urease is in a free state, it lacks nucleation sites compared with MICP technology, which affects the deposition of calcium carbonate, resulting in deviations in the overall effect of EICP on soil reinforcement.

Method used

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  • Method for reinforcing soil body by combination of organic matter and plant urease
  • Method for reinforcing soil body by combination of organic matter and plant urease
  • Method for reinforcing soil body by combination of organic matter and plant urease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for improving soil by organic matter improving soybean urease to induce calcium carbonate, comprising the following steps:

[0034] (1) Soybean powder preparation: Soybeans need to be baked in an oven at 40°C for 6 hours, crushed by a crusher, and passed through a 100-mesh sieve to obtain soybean powder;

[0035] (2) Soybean urease extraction: Add soybean powder and deionized water to the conical flask at a mass concentration of 100g / L to obtain soybean liquid, heat the soybean liquid to 50°C with electromagnetic stirring, and centrifuge at 3500r / min for 30min; filter Remove the bean dregs, leave the remaining solution in the refrigerator for 12 hours, and obtain the supernatant, which is the soybean urease solution;

[0036] (3) Adding organic matter: Add skimmed milk powder into the soybean urease solution at a rate of 4g / L to obtain an organic matter urease mixture;

[0037] (4) Preparation of cementing liquid: CaCl 2 and CO(NH) 2 Mix according to the con...

Embodiment 2

[0040] The difference from Example 1 is that the organic matter mixed in step (3) is 8 g / L skimmed milk powder.

Embodiment 3

[0042] The difference from Example 1 is that the organic matter mixed in step (3) is 16 g / L skimmed milk powder.

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Abstract

The invention provides a method for reinforcing a soil body by a combination of organic matter and plant urease. The method includes the following steps of firstly, extracting the soybean urease; secondly, mixing the organic matter such as glutinous rice flour, brown sugar or skim milk powder into a solution of soybean urease in proportion; and thirdly, sequentially injecting the organic matter and urease mixed liquid and consolidating fluid into the soil body in certain proportion. According to the method, nucleation point locations are provided for calcium carbonate precipitation by introducing the organic matter into a urease induced calcium carbonate precipitation (EICP) technology so that calcium ions can be concentratedly adsorbed to anion groups provided by the organic mater and large-grained calcium carbonate crystals can aggregate at the nucleation point locations; the growth habit of mineral crystals is affected, the morphology structure of minerals is improved, and the strength of soil body is greatly improved and enhanced; and the activity of the urease is protected, and the calcium carbonate precipitation efficiency under a high concentration is slightly improved. Themethod is efficient, economical, simple in process, friendly to the environment, convenient to popularize and suitable for large-scale treatment.

Description

technical field [0001] The invention belongs to the technical fields of foundation treatment and geotechnical engineering reinforcement, and in particular relates to a method for reinforcing soil with organic matter combined with plant urease. Background technique [0002] MICP (Microbial Induced Calcium Carbonate Deposition) has become a new type of soil reinforcement technology in recent years because of its environmental friendliness and low energy consumption. However, affected by the size and aerobicity of urease-producing bacteria, the type and depth of soil treated by MICP are limited to a certain extent; and the microbial cultivation steps are complicated and difficult to control, and there is still the problem of secondary pollution by by-products. The researchers found that urease can not only be secreted by microorganisms, but also be extracted from cheap plants, and then proposed a technology of directly using urease to induce calcium carbonate deposition (EICP)....

Claims

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Application Information

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IPC IPC(8): E02D3/12C09K17/14C09K103/00
CPCC09K17/14C09K2103/00E02D3/12
Inventor 原华任冠洲赵志亮原佩赵恒亮原耀楠朱红云刘康张建伟
Owner HENAN UNIVERSITY
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