Method for reinforcing soil body by combination of organic matter and plant urease

A technology of plant urease and organic matter, which is applied in geotechnical engineering reinforcement and foundation treatment, can solve the problems of EICP reinforcement soil overall effect deviation, lack of nucleation sites, and influence on calcium carbonate deposition, so as to improve mineral morphology and structure, Resource rich, effects that increase strength and effect

Inactive Publication Date: 2020-10-27
HENAN UNIVERSITY
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  • Description
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  • Application Information

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Problems solved by technology

However, since plant urease is in a free state, it lacks nucleation sites compared with MICP technology, which affec

Method used

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  • Method for reinforcing soil body by combination of organic matter and plant urease
  • Method for reinforcing soil body by combination of organic matter and plant urease
  • Method for reinforcing soil body by combination of organic matter and plant urease

Examples

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[0032] Example 1

[0033] A method for organically modified soybean urease to induce calcium carbonate to strengthen soil, including the following steps:

[0034] (1) Soy flour preparation: Soybeans need to be put into an oven and roasted at 40℃ for 6 hours, crushed by a crusher and passed through a 100-mesh sieve to obtain soybean flour;

[0035] (2) Soybean urease extraction: add soybean powder and deionized water to an Erlenmeyer flask at a mass concentration of 100g / L to prepare soybean liquid, heat the soybean liquid to 50°C with electromagnetic stirring, and centrifuge at 3500r / min for 30min; filter; Remove the bean dregs, and let the remaining solution stand in the refrigerator for 12 hours, and the supernatant obtained is the soybean urease solution;

[0036] (3) Blending organic matter: 4g / L of skimmed milk powder is blended into the soybean urease solution to prepare an organic matter urease mixture;

[0037] (4) Preparation of cementing liquid: CaCl 2 And CO(NH) 2 Mix by con...

Example Embodiment

[0039] Example 2

[0040] The difference from Example 1 is that the organic matter incorporated in step (3) is 8g / L skimmed milk powder.

Example Embodiment

[0041] Example 3

[0042] The difference from Example 1 is that the organic matter blended in step (3) is 16 g / L skimmed milk powder.

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Abstract

The invention provides a method for reinforcing a soil body by a combination of organic matter and plant urease. The method includes the following steps of firstly, extracting the soybean urease; secondly, mixing the organic matter such as glutinous rice flour, brown sugar or skim milk powder into a solution of soybean urease in proportion; and thirdly, sequentially injecting the organic matter and urease mixed liquid and consolidating fluid into the soil body in certain proportion. According to the method, nucleation point locations are provided for calcium carbonate precipitation by introducing the organic matter into a urease induced calcium carbonate precipitation (EICP) technology so that calcium ions can be concentratedly adsorbed to anion groups provided by the organic mater and large-grained calcium carbonate crystals can aggregate at the nucleation point locations; the growth habit of mineral crystals is affected, the morphology structure of minerals is improved, and the strength of soil body is greatly improved and enhanced; and the activity of the urease is protected, and the calcium carbonate precipitation efficiency under a high concentration is slightly improved. Themethod is efficient, economical, simple in process, friendly to the environment, convenient to popularize and suitable for large-scale treatment.

Description

technical field [0001] The invention belongs to the technical fields of foundation treatment and geotechnical engineering reinforcement, and in particular relates to a method for reinforcing soil with organic matter combined with plant urease. Background technique [0002] MICP (Microbial Induced Calcium Carbonate Deposition) has become a new type of soil reinforcement technology in recent years because of its environmental friendliness and low energy consumption. However, affected by the size and aerobicity of urease-producing bacteria, the type and depth of soil treated by MICP are limited to a certain extent; and the microbial cultivation steps are complicated and difficult to control, and there is still the problem of secondary pollution by by-products. The researchers found that urease can not only be secreted by microorganisms, but also be extracted from cheap plants, and then proposed a technology of directly using urease to induce calcium carbonate deposition (EICP)....

Claims

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Application Information

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IPC IPC(8): E02D3/12C09K17/14C09K103/00
CPCC09K17/14C09K2103/00E02D3/12
Inventor 原华任冠洲赵志亮原佩赵恒亮原耀楠朱红云刘康张建伟
Owner HENAN UNIVERSITY
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