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Hosta undulata bailey test-tube plantlet preservation culture medium and application thereof

A technology of hosta and culture medium, which is applied in the field of storage medium for test-tube seedlings of hosta, which can solve the problems of degradation, variation, and short storage period of test-tube seedlings, and achieve the effects of simple operation, reduced storage costs, and low cost

Active Publication Date: 2020-10-30
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The culture medium of the present invention aims at the short storage period in the prior art, the need for frequent subculture renewal, and multiple subcultures can easily lead to the degradation or variation of test-tube plantlets, which can make up for the deficiencies in the prior art. Hosta mosaic test-tube plantlet medium

Method used

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  • Hosta undulata bailey test-tube plantlet preservation culture medium and application thereof
  • Hosta undulata bailey test-tube plantlet preservation culture medium and application thereof
  • Hosta undulata bailey test-tube plantlet preservation culture medium and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 method for preserving test-tube seedlings of hosta mosaic

[0029] Experimental materials: The test-tube seedlings of Hosta mosaic came from the test-tube seedling bank of the National Crop Germplasm Bank.

[0030] The specific steps of the experimental method are as follows:

[0031] (1) The growth method after the test-tube plantlet is multiplied is as follows: the aseptic test-tube plantlet of the hosta mosaic that has been multiplied and multiplied, under the conditions of a culture temperature of 20-25°C, a light intensity of 2000-3000lx, and a light time of 12-14h / d , into MS+3% sucrose+0.7% agar (pH5.8) culture medium for 30-45 days

[0032](2) The delayed growth preservation method is as follows: Select the aseptic test-tube plantlets of Hosta mosaic with 4 leaves and 1 heart (5 ± 0.5 cm in height) that are robust and consistent in growth in step (1) and inserted into the delayed growth preservation medium MS+ 50g / L sucrose + 20g / L mannitol + 1.0m...

Embodiment 2

[0035] The survival rate of embodiment 2 hosta test-tube plantlets preserved after 6 months

[0036] Take the aseptic seedlings of Hosta mosaic that grow vigorously and grow uniformly (4 leaves and 1 heart, height is 5 ± 0.5cm) after being cultivated on MS+3% sucrose+0.7% agar (pH5.8) medium for 30 to 45 days. The materials were respectively inserted into the medium of 11 kinds of preservation schemes, and the culture conditions were the same as in Example 1, and the influence of the culture medium of each preservation scheme on the survival rate was studied. The results are shown in Table 1.

[0037] Table 1 The survival rate of hosta mosaic in each preservation scheme for 6 months

[0038]

[0039]

[0040] As can be seen from Table 1, although the survival rate of the control group was still 100% when preserved for 3 months, the test-tube plantlets had been topped with bottle caps and there was little left in the culture medium, so it was necessary to subculture. Re...

Embodiment 3

[0041] Example 3 Changes in Plant Height in Delayed Growth and Preservation of Hosta Mosaic Test-tube Plantlets

[0042] (1) grow robustly and grow uniformly (4 leaves and 1 heart, height is 5 ± 0.5cm) after cultivating on MS+3% sucrose+0.7% agar (pH5.8) medium for 30 to 45 days. Bacteria were used as materials, which were respectively inserted into the control medium (MS+3% sucrose+0.7% agar, pH5.8) and the medium of the delayed growth preservation scheme (MS+50g / L sucrose+20g / L mannitol+1.0mg / L abscisic acid (ABA)+20mg / L chlormequat (CCC)+0.7% agar, pH5.8) is the medium in the A scheme of embodiment 2, and culture condition is the same as embodiment 1, researches in embodiment 2 Effect of protocol A medium on plant height of surviving plants. The results are shown in Table 2, figure 1 , figure 2 shown.

[0043] Table 2 Plant height changes of Hosta mosaic during preservation

[0044]

[0045] It can be seen from Table 2 that with the prolongation of storage time, t...

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Abstract

The invention discloses a hosta undulata bailey test-tube plantlet preservation culture medium and application thereof, and belongs to the technical field of plant tissue culture. The hosta undulata bailey test-tube plantlet preservation culture medium is an MS culture medium comprising cane sugar, mannitol, abscisic acid, chlormequat chloride and agar. The culture medium disclosed by the invention is used for preserving test-tube plantlets of the hosta undulata bailey, the test-tube plantlets of the hosta undulata bailey can be preserved for 6 months, the survival rate of the preserved material is 100%, and the plant is stable in phenotype and free of degeneration and variation after being recovered and cultured. The culture medium is low in cost, overcomes the defects that an existing preservation culture medium is short in preservation time and phenotypes are prone to variation after preservation, can be applied to preservation of hosta undulata bailey germplasm resources and is huge in application value.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a medium for preserving test-tube seedlings of hosta mosaicus and its application. Background technique [0002] Hosta plantaginea is a perennial herbaceous plant of the Liliaceae Hosta genus. It is a variant of the famous foliage plant Hosta. It has strong complementarity to light-absorbing flowers and is an excellent variety for landscaping. The plants of Hosta mosaic are dense, the leaves are ovate to heart-shaped, with yellow streaks, and there are many cultivars. The leaf shape, leaf color and plant type are very different. The green ones are verdant, and the blue ones are faint. Elegant, brilliantly colored foliage in magical gradations of color. In the upsurge of cultivating new varieties of the genus Hosta in the world, Hosta mosaic stands out among the hosta varieties because of its unique leaf color. Therefore, Hosta mosaic has become a wild f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/001A01H4/008A01N3/00
Inventor 陈晓玲张金梅李洋卢新雄辛霞尹广鹍何娟娟刘运霞黄雪琦
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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