Method for detecting high-density lipoprotein subcomponent in preeclampsia judgment process

Abstract

The invention discloses a method for detecting a high-density lipoprotein subcomponent in a preeclampsia judgment process, which comprises the following steps: presetting a high-density lipoprotein HDL subcomponent analysis kit, obtaining a blood sample, and detecting the to-be-detected blood sample by using the high-density lipoprotein HDL subcomponent analysis kit; carrying out standing centrifugal treatment on the blood sample, separating out plasma/serum, and adding the plasma/serum sample into a sample buffer solution containing lipophilic fluorescent dye, wherein the dye can be specifically combined with lipoprotein; injecting a gel dye into a microfluidic electrophoresis chip, adding a to-be-detected blood sample, performing detecting on a microfluidic electrophoresis chip analyzer,sending the sample into a separation channel under the action of an external electric field and electroosmotic flow, and separating HDL in the to-be-detected sample into a plurality of sub-componentsunder the action of a molecular sieve and charges. According to the structure, the judgment accuracy of preeclampsia is improved.

Description

technical field [0001] The invention relates to the technical field of medical analysis, in particular to a method for detecting high-density lipoprotein subcomponents in the process of judging preeclampsia. Background technique [0002] HDL is a group of heterogeneous particles of different sizes and components, containing approximately equal amounts of lipids (cholesterol and phospholipids) and proteins, mainly secreted by the liver and small intestine, of which 70% to 80% come from the liver and are circulating The main source of HDL in the system. The density of HDL particles ranges from 1063 to 1210 g / L, and the diameter is about 5 to 17 nm. According to the differences in the lipids, apolipoprotein (Apo), enzymes and lipid transport proteins contained, and the differences in the density, size and charge of the particles between the subgroups, different physical and chemical methods can be used to produce different Subgroup classification methods, such as: according t...

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Problems solved by technology

[0006] In addition, the recruitment of neutrophils activated by the complement system at the maternal-fetal interface will lead to an increase in the level of tumor necrosis factor-α (tumor necrosis factor alpha, TNF-α), and excessive deposition of antigen-antibody reactive complement around the capillaries , and then lead to the decrease of angiogenesis factors such as VEGF, resulting in the disorder of spiral artery remodeling, resulting in poor placenta formation and maternal blood vessel hypoperfusion, and induces the occurrence of preeclampsia

Eventually lead to placenta formation and miscarriage

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