Method for establishing plant regeneration system in Iris laevigata cluster bud way

Abstract

The invention provides a method for establishing a plant regeneration system in an Iris laevigata cluster bud way. The method comprises the following steps: (1) disinfecting seeds, namely disinfectingmature Iris laevigata seeds by using alcohol and a NaClO solution; (2) obtaining explants, namely germinating the disinfected seeds in sterile water, and taking root-removed base growth points of seedlings with 2-3 primary leaves after seed germination as the explants; (3) performing cluster bud induction and multiplication culture, namely inoculating the explants into a cluster bud induction andmultiplication culture medium, and replacing the culture medium once every other 30 days; (4) performing rooting culture on tissue culture seedlings, namely when the cluster buds grow to 5-10cm, cutting and trimming the cluster buds, and inoculating the cluster buds into a rooting culture medium; and (5) hardening seedlings and performing transplanting, namely hardening the rooted plants in a room for 3 days, performing water culture for 20 days, and transplanting the plants into a flowerpot filled with a culture medium (the ratio of garden soil to vermiculite is 3:1). According to the method, tissue culture rapid propagation of Iris laevigata is realized, resource development and utilization are promoted, and the Iris laevigata germplasm resources can be effectively protected.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for establishing a plant regeneration system of a swallow flower cluster bud approach. Background technique [0002] Iris laevigata (Iris laevigata), also known as flat-leaf iris and light-leaved iris, is a perennial herb of the family Iridaceae. It is mostly distributed in Heilongjiang, Jilin, Liaoning and Yunnan in China, and is also produced in Japan, Korea and Russia. It is suitable for growing in swamps , water wetlands on the banks of the river. Swallow flower has been introduced and applied in botanical gardens all over the world, and is a famous ornamental flower. It has bright colors, elegant flower posture, tall and straight leaves, high ornamental value, strong group function, long cultivation history, excellent cold resistance, and can adapt to a variety of habitats. It is an excellent material for decorative environments and has various garden ...

AI Technical Summary

Problems solved by technology

[0005] At present, the traditional propagation technology of Swallows flower has the disadvantages of low seed germination rate, slow propagation, and low ramet reproduction coefficient; the known plant regeneration system of Swallows flower is obviously restricted by seasons, and there is no clear report on the rooting rate and rooting coefficient of adventitious buds in the regeneration system. The establishment method of the plant regeneration system of Swallow flower still needs to be perfected

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