A method for establishing a plant regeneration system of a swallow flower cluster bud approach

A method of establishment, a technology of swallows, applied in the field of plant tissue culture, can solve the problems of no adventitious bud rooting rate and rooting coefficient, low division reproduction coefficient, low seed germination rate, etc., to meet the needs of garden applications and shorten the breeding cycle , Guarantee the effect of excellent properties

Active Publication Date: 2022-06-07
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

[0005] At present, the traditional propagation technology of Swallows flower has the disadvantages of low seed germination rate, slow propagation, and low ramet reproduction coefficient; the known plant regeneration system of Swallows flower is obviously restricted by seasons, and there is no clear report on the rooting rate and rooting coefficient of adventitious buds in the regeneration system. The establishment method of the plant regeneration system of Swallow flower still needs to be perfected

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  • A method for establishing a plant regeneration system of a swallow flower cluster bud approach
  • A method for establishing a plant regeneration system of a swallow flower cluster bud approach
  • A method for establishing a plant regeneration system of a swallow flower cluster bud approach

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[0029] The method of the present invention will be described in further detail below with reference to specific embodiments and accompanying drawings.

[0030] The method for establishing a plant regeneration system of the swallowtail flower cluster bud approach provided by the present embodiment comprises the following steps:

[0031] (1) Disinfection of seeds: remove the outer seed coat of the mature swallow flower seeds and put them in a conical flask, soak them in diluted detergent for 10 minutes to remove impurities on the surface of the seeds, rinse them under running water for 25 minutes until no foam is generated, and then immerse them in an ultra-high temperature Put the rinsed seeds into a sterilized conical flask in the clean workbench, disinfect with 75% alcohol for 10s, rinse with sterile water for 3 times, add 2% NaClO solution to soak for 25min, and rinse with sterile water for 5 minutes. During the whole disinfection process, the disinfection container should b...

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Abstract

The invention provides a method for establishing a plant regeneration system of the bud-forming approach of Swallows japonica, comprising the following steps: (1) seed disinfection: the mature Swallows swan seeds are sterilized with alcohol and NaClO solution; Germinate in bacterial water, use the basal growth points of seedlings with 2-3 primary leaves after seed germination to remove the roots as explants; (3) cluster bud induction and proliferation culture: inoculate explants in cluster bud induction proliferation culture In the medium, replace the medium every 30d; (4) rooting culture of tissue culture seedlings: when the clustered buds grow to 5-10cm, they are divided and trimmed, and inoculated in the rooting medium; (5) hardening and transplanting: rooting After 3 days of hardening indoors, the plants were transplanted into flower pots equipped with a cultivation medium (garden soil: vermiculite = 3:1) after 20 days of hydroponic cultivation. The method realizes the tissue culture and rapid propagation of the Swallows flower, promotes resource development and utilization, and can effectively protect the Swallows flower germplasm resource.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for establishing a plant regeneration system for the budding pathway of Swallow Flower. Background technique [0002] Swallow flower (Iris laevigata), also known as flat-leaf iris and light-leaf iris, is a perennial herb of the genus Iridaceae. It is mostly distributed in Heilongjiang, Jilin, Liaoning and Yunnan in China, as well as in Japan, Korea and Russia. It is suitable for growing in swampy land. , Wetlands on the banks of the river. Swallow flower has been introduced and applied in botanical gardens all over the world, and it is a famous ornamental flower. It has bright flower color, elegant flower posture, tall and straight leaves, high ornamental value, strong group function, long history of cultivation, excellent cold resistance, and can adapt to various habitats. It is an excellent material for decorative environments and has various forms of gar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王玲李凤仪刘玉佳
Owner NORTHEAST FORESTRY UNIVERSITY
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