Gene detection reagent for predicting liver injury caused by antituberculous drug, test kit and detection method

A technology of genetic detection and detection method, applied in the field of tuberculosis, can solve the problem of lack of risk prediction of adverse reactions

Inactive Publication Date: 2020-11-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of this, one of the purposes of the present invention is to provide a reagent for predicting the detection of liver injury genes caused by anti-tuberculosis drugs, which solves the lack of adverse reactions to tuberculosis patients in the prior art after receiving anti-tuberculosis drugs. Technical Issues in Risk Forecasting

Method used

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  • Gene detection reagent for predicting liver injury caused by antituberculous drug, test kit and detection method
  • Gene detection reagent for predicting liver injury caused by antituberculous drug, test kit and detection method
  • Gene detection reagent for predicting liver injury caused by antituberculous drug, test kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Mining of susceptibility SNPs loci for liver injury induced by anti-tuberculosis drugs

[0042] 1. Inclusion of TB population in western China

[0043] A total of 1060 highly suspected tuberculosis patients who were treated at West China Hospital of Sichuan University from December 2016 to April 2018 were continuously included. According to the typical tuberculosis symptoms, microbiology and imaging results to diagnose tuberculosis patients, and excluding patients with liver disease and poor compliance during treatment or follow-up lost contact, this example finally included 745 tuberculosis patients, see figure 1 .

[0044] The inclusion and exclusion criteria are as follows:

[0045] Inclusion criteria: 1) have typical symptoms and signs of tuberculosis; 2) positive acid-fast stained smears or positive Mycobacterium tuberculosis culture or positive nucleic acid test; 3) imaging data showing typical evidence of active tuberculosis lesions; 4) disease The physical re...

Embodiment 2

[0081] This embodiment provides a method for detecting the genotypes of rs2025804 and rs2104564, which is a small sequencing method, that is, a single base extension termination method.

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Abstract

The invention provides a gene detection reagent for predicting liver injury caused by antituberculous drug, a test kit and a detection method, and relates to the technical field of tuberculosis treatment. The reagent is used for predicting the risk of adverse liver reaction caused by the antituberculous drug received by tuberculosis patients. The reagent comprises a genotype used for detecting anyone of the following SNPs sites of the LEPR gene: rs2025804 and rs2104564. According to the present invention, with the detection of the polymorphism of the LEPR gene, the risk of the adverse reaction after the tuberculosis patient receives the anti-tuberculosis drug treatment can be predicted, the certain support is provided for the prevention of the adverse reaction caused by the related anti-tuberculosis drug, and the certain guidance is provided for the medication of the tuberculosis patient.

Description

technical field [0001] The invention relates to the technical field of tuberculosis, in particular to a reagent, a kit and a detection method for predicting the detection of liver damage genes caused by anti-tuberculosis drugs. Background technique [0002] Tuberculosis (TB) is a major infectious disease that seriously endangers human health. According to the World Health Organization (WHO) Global Tuberculosis Annual Report, in 2017, about 10 million people were newly infected with tuberculosis in the world, and about 889,000 people were newly infected with tuberculosis in my country, making it the second largest country with a high burden of tuberculosis in the world. Tuberculosis accounted for the second largest number of legal infectious diseases in my country in 2017, and the second highest mortality rate. The situation of anti-tuberculosis treatment is still very severe. The cornerstone of tuberculosis treatment is the standardized anti-tuberculosis regimen recommended...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 吴涛应斌武赵珍珍
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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