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Seed production method for improving over-summer survival rate of crassostrea gigas

A technology for growing oysters and survival rate, which is applied in the field of seed production for improving the survival rate of oysters during the summer, can solve the problems of low survival rate during the summer, and achieves the effects of improving the breeding efficiency, strong practicability and easy popularization.

Active Publication Date: 2020-12-01
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of low summer survival rate in the current long oyster industry, the present invention provides a seed production method that can significantly increase the summer survival rate of long oyster in summer and provide important technical support for its new variety cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Parent selection: About 5,000 wild long oysters were collected in Aoshan Bay, Jimo District, Qingdao. When the seawater temperature was 4-8°C, oysters with a shell height greater than 5cm and rudimentary gonads were selected as alternative parents and placed in the Institute of Oceanology, Chinese Academy of Sciences. Shellfish farming laboratory.

[0019] The oysters were separated into monomers and the dirt on the surface of the shells was removed, and filtered seawater was used to adapt to indoor natural water temperature conditions for 14 days. Put the oysters in seawater with a water temperature of 42°C for 1 hour after high-temperature stress treatment, and make the parents experience the temperature stress from low temperature to extreme high temperature in a short period of time, so as to quickly purify the parents, and then put them in natural seawater, and pick them to death every 12 hours Individuals have no dead individuals after 14 days, and after 72 hours ...

Embodiment 2

[0025] Parent selection: Collect wild long oysters in the subtidal zone of Aoshan Bay, Jimo District, Qingdao. When the seawater temperature is 4-8°C, select oysters with a shell height greater than 5cm and undeveloped gonads as alternative parents and put them in the Institute of Oceanology, Chinese Academy of Sciences shellfish farming laboratory.

[0026] The oysters were separated into monomers and the dirt on the surface of the shells was removed, and filtered seawater was used to adapt to indoor natural water temperature conditions for 14 days. After 1726 oysters were placed in seawater at a water temperature of 42°C for 1 hour of high-temperature stress treatment, the parents were subjected to temperature stress from low temperature to extreme high temperature in a short period of time, so as to quickly purify the parents, and then placed in natural seawater. The statistical survival rate was 37.19% after picking dead individuals once every 12 hours until there were no ...

Embodiment 3

[0031] Parent selection: Collect about 3,000 wild long oysters in the super subtropical sea area of ​​Qingdao. When the seawater temperature is 4-8°C, select oysters with a shell height greater than 5cm and undeveloped gonads as alternative parents and put them in the Institute of Oceanology, Chinese Academy of Sciences. Shellfish farming laboratory.

[0032] The oysters were separated into monomers and the dirt on the surface of the shells was removed, and filtered seawater was used to adapt to indoor natural water temperature conditions for 14 days. Put the oysters in seawater with a water temperature of 41°C for 2 hours after high-temperature stress treatment, and make the parents experience the temperature stress from low temperature to extreme high temperature in a short period of time, so as to quickly purify the parents, then put them in natural seawater, and pick them to death every 12 hours Individuals have no dead individuals after 14 days, and after 72 hours without...

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Abstract

The invention belongs to the field of shellfish genetic breeding in marine biotechnology, and particularly relates to a seed production method for improving the over-summer survival rate of crassostrea gigas. The method comprises the steps of parent selection, breeding line construction, juvenile mollusk optimization, germplasm purification and the like. According to the method, the resistance characteristics of the oysters in different development stages are utilized, and the oysters are efficiently screened by adopting two extremely acute environmental stresses of dry dew and high temperature in the juvenile mollusk stage and the adult mollusk stage respectively, so that the breeding efficiency is improved, the breeding period is shortened, and the over-summer survival rate of the oysters is remarkably increased. The method has the advantages of being high in practicability, remarkable in effect and easy to popularize. By means of the method, the oyster new strain germplasm with thehigh over-summer survival rate can be obtained, and the system risk of the oyster breeding industry is remarkably reduced.

Description

technical field [0001] The invention relates to the field of shellfish genetics and breeding in marine biotechnology, in particular to a seed production method for improving the summer survival rate of long oysters. Background technique [0002] Long oyster is a traditional cultured shellfish in northern my country, and it is also the oyster species with the largest breeding volume and the widest distribution in the world. Long oysters are usually cultured in open seas, where environmental factors cannot be intervened by humans. In the past ten years, the mass mortality of long oysters in summer has occurred frequently all over the world, and the mortality rate in some countries’ sea areas can reach 50%-90%, which poses a major threat to oyster production and is a bottleneck factor for the stable and healthy development of the oyster industry. . Improving the summer survival rate of long oyster is the focus of the oyster industry. However, due to the complex factors affect...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 丛日浩李莉张国范丁芳芳赵泽民王威王鲁平
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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