Marker combination, kit and application thereof for detection of early gastric cancer

Abstract

The present invention relates to a marker combination, kit and application for early gastric cancer detection, the early gastric cancer marker combination includes the following miRNAs detectable in human serum: hsa-miR-17-5p, hsa-miR-5p 18a‑5p, hsa‑miR‑19b‑3p, hsa‑miR‑20a‑5p, hsa‑miR‑21‑5p, hsa‑miR‑25‑5p, hsa‑miR‑27a‑5p, hsa‑miR‑29c‑ 5p, hsa‑miR‑93‑5p, hsa‑miR‑100‑5p, hsa‑miR‑103a‑3p, hsa‑miR‑106a‑5p, hsa‑miR‑106b‑5p, hsa‑miR‑148a‑5p, hsa‑miR‑183‑5p, hsa‑miR‑199a‑3p, hsa‑miR‑218‑5p, hsa‑miR‑222‑5p, hsa‑miR‑337‑3p, hsa‑miR‑365a‑3p, hsa‑miR‑218‑5p, hsa‑miR‑222‑5p, hsa‑miR‑337‑3p, hsa‑miR‑365a‑3p miR‑423‑5p, hsa‑miR‑425‑5p, hsa‑miR‑486‑5p, hsa‑miR‑487b‑3p, hsa‑miR‑532‑3p, hsa‑miR‑590‑5p, hsa‑miR‑ 615‑3p, hsa‑miR‑744‑5p. Compared with the existing miRNA marker combination, the miRNA marker combination obtained in the present invention covers a wider range of miRNAs and has a higher detection ability for early gastric cancer. The detection reliability has been independently verified by two verification groups, and based on two The experimental cost of generational sequencing is also within an acceptable range.

Description

technical field [0001] The invention relates to a marker combination, kit and application for early gastric cancer detection, and belongs to the field of biotechnology. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world, and it is also one of the malignant tumors with the highest mortality rate. According to statistics in recent years, in China, gastric cancer has long been one of the three major causes of death from various types of cancer. At present, significantly improved surgical techniques and various newly developed treatment methods have significantly improved the treatment effect and survival time of patients with early gastric cancer; however, the survival time of patients with advanced gastric cancer is still not optimistic. The five-year survival rate is far below 50%, and the high cost of long-term treatment has brought a heavy burden to the family and society. Therefore, early detection of gastric cancer is cr...

AI Technical Summary

Problems solved by technology

However, there are still some deficiencies in current research on circulating miRNAs as diagnostic markers for gastric cancer. For example, (1) a large part of research only selects miRNAs that have been reported to be dysregulated in gastric cancer tissues as candidate indicators, and these miRNAs are detected in serum is not necessarily the optimal choice, and (2) some studies have used microarray for preliminary screening of miRNA markers, but compared with next-generation sequencing, the signal-to-noise ratio of microarray is poor, so the miRNA markers screened out is not necessarily preferred

Images