Efficient artificial spawning induction method for cynoglossus semilaevis female fish

Abstract

The invention belongs to the technical field of aquaculture, and relates to an efficient artificial spawning induction method for cynoglossus semilaevis female fish. According to the method, efficientartificial spawning induction of cynoglossus semilaevis female parent fish is achieved through selection of the female parent fish, control over spawning induction pond culture conditions, artificialspawning induction, artificial insemination and incubation. By the adoption of the method, the spawning induction rate, the relative spawning amount, the fertilization rate and the hatching rate of the cynoglossus semilaevis female fish are greatly increased, and the method is an extremely efficient artificial spawning induction method. The method provided by the invention has very good practicability and operability, and is beneficial to further rapid development of the cynoglossus semilaevis culture industry.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to a highly efficient method for artificially inducing labor of a half-smooth tongue sole female. Background technique [0002] Cynoglossus semiliaevis (Cynoglossus semiliaevis) belongs to the Plaiceidae, Cynoglossus semiliaevis, Cynoglossus semiliaevis, commonly known as Cynoglossus semiliaevis, and is one of the important marine fishes in coastal areas of my country. Semi-smooth tongue sole artificial breeding technology was successful in 2002, and then the domestic breeding scale gradually expanded. Because of its delicious taste and rich nutrition, it is popular with consumers, its economic value is extremely high, and its breeding prospects are very broad. [0003] In recent years, the average annual seed demand has continued to rise. However, due to the limitation of artificial induced spawning technology, the existing artificial induced spawning technology for female brood...

AI Technical Summary

Problems solved by technology

[0003] In recent years, the average annual seed demand has continued to rise. However, due to the limitation of artificial induced spawning technology, the existing artificial induced spawning technology for female broodstock of semi-smooth tongue sole has a low success rate, low egg production, fertilization rate and hatching rate, and is extremely unstable.

For example, in the method reported by Yang Jingfeng et al. (Research on Artificial Oxygenation Technology of Semi-smooth Tongue Sole, Journal of Inner Mongolia University for Nationalities, 2010), 10 different formulas were used to artificially induce labor for 10 semi-smooth tongue sole females. The parent fish did not conduct water temperature and light before and after induced labor. Intensity-adjusted stimulation, the overall fertilization rate is 65.3%, and the overall hatching rate is 44.8%. This method has major defects, that is, it has not formed a stable and reliable artificial oxytocin technology and method, and it is difficult to apply it on a large scale in production

Another example is the method in the Chinese invention patent "Method for Obtaining Fertilized Eggs of Semi-smooth Tongue Sole Throughout the Year" (CN202010506606.8), by using luteinizing hormone releasing hormone analog (LHRH-A3) plus chorionic gonadotropin (HCG) to Semi-smooth tongue sole broodstock was artificially induced (once), the core of which is that this method achieves high female fertilized egg production throughout the year. There is no special instruction on the water temperature and light adjustment before and after induction. The egg production and fertilization rate of this method And the hatching rate is not high, there is also the problem of low efficiency

In addition, the method reported in Zhang Yuxiang et al. (Effects of oxytocin on ovarian biochemical components and plasma reproductive hormone levels in half-smooth tongue sole, Aquatic Sciences, 2017) used luteinizing hormone-releasing hormone analog (LHRH-A3) plus chorionic gonadotropin ( HCG) artificially induced labor for half-smooth tongue sole females. Although this method uses the method of secondary induction, this paper mainly focuses on the research on the biochemical components of the half-smooth tongue sole ovary and plasma reproductive hormones after the induction, although the amount of eggs laid, the number of floating eggs, etc. However, the three important indicators to measure the effect of oxytocin, such as oxytocin rate, fertilization rate and hatching rate, have not been reported, so the application effect of the method in this paper is unknown

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