Method for molecular marker-assisted breeding of super-early-maturing cotton germplasm

A molecular marker-assisted, super-early-maturing technology, applied in biochemical equipment and methods, proteomics, microbial measurement/inspection, etc., can solve the problems of low breeding efficiency of early-maturing cotton, improve breeding efficiency and success rate, and solve The effect of low breeding efficiency

Active Publication Date: 2021-02-09
邯郸市农业科学院
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Problems solved by technology

[0007] The invention proposes a molecular marker-assisted method for breeding extra-early-maturing cotton germplasm, which solves the problem of low breeding efficiency of early-maturing cotton in the prior art

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  • Method for molecular marker-assisted breeding of super-early-maturing cotton germplasm
  • Method for molecular marker-assisted breeding of super-early-maturing cotton germplasm
  • Method for molecular marker-assisted breeding of super-early-maturing cotton germplasm

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Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0023] Such as figure 1 As shown, a method for molecular marker-assisted breeding of extra-early-maturing cotton germplasm, comprising the following steps:

[0024] S1. A total of 140 cotton varieties with at least one trait in early maturity, high yield, disease resistance, and high-quality traits were collected in the cotton area of ​​the Yellow River Basin, as shown in Table 1, as a high-quality resource bank for parent selection;

[0025] Table 1 140 high-quality cotton varie...

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Abstract

The invention belongs to the technical field of cotton breeding, and provides a method for molecular marker-assisted breeding of extra-early-maturing cotton germplasm, which comprises the following steps: S1, collecting a cotton variety with at least one of early maturing, high yield, disease resistance and high quality as a high-quality resource library; S2, extracting DNA of each cotton variety,synthesizing an SRAP primer, and calculating a Jaccard similarity coefficient and carrying out clustering analysis by utilizing an SRAP molecular marker; s3, screening out candidate parents in combination with specific primer character identification; S4, grouping the candidate parents, carrying out an incomplete biserial hybridization test, and calculating general combining ability and special combining ability; s5, preparing a hybridized combination, and breeding to obtain a stable strain; and S6, detecting the early-maturing gene of the new strain by using the specific primers of the SSR molecular marker to obtain the early-maturing, high-yield, high-quality and disease-resistant strain. By means of the technical scheme, the problem that in the prior art, early-maturing cotton breedingefficiency is low is solved.

Description

technical field [0001] The invention belongs to the technical field of cotton breeding and relates to a method for molecular marker-assisted breeding of extra-early-maturing cotton germplasm. Background technique [0002] Cotton is a global cash crop that plays an important role in several industries including textile industry, food industry and defense industry. Among the four cotton cultivars, upland cotton is currently the most widely cultivated cotton in the world, and its output accounts for more than 90% of the world's total cotton output. According to the length of growth period, upland cotton can be divided into: early-maturing varieties (growing period ≤ 120 days, also known as short-season cotton), medium-early-maturing varieties (growing period 121-130 days), medium-maturing varieties (growing period 131-140 days), Mid-late maturing varieties (growing period 141-150 days) and late-maturing varieties (growing period > 150 days). With the development of human b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B40/00G16B20/30G06K9/62C12Q1/6895
CPCG16B40/00G16B20/30C12Q1/6895C12Q2600/156C12Q2600/13G06F18/23
Inventor 张彦波路正营刘红耀孙璐李世云尹国杨玉枫
Owner 邯郸市农业科学院
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