Method for breeding salt-tolerant lily new strain by using EMS reagent mutagenesis
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A new strain, salt-tolerant technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of slow progress of salt-tolerant strains, restricting the development of lily industry, and lack of germplasm resources.
Inactive Publication Date: 2021-03-12
LIANYUNGANG ACAD OF AGRI SCI
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Problems solved by technology
Soil salinity is the key factor related to the normal growth of lily, but in most parts of northern China, the water and soil are slightly alkaline, which greatly limits the development of lily industry.
[0003] At present, the research on salt-tolerant varieties of lily mainly focuses on the evaluation of salt-tolerant systems and field screening, but there are problems such as few germplasm resources and slow progress of salt-tolerant strains, which are difficult to meet the needs of lily industry development
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Embodiment 1
[0038] Example 1 Variety "Sorbonne"
[0039] Utilize EMS reagent mutagenesis to carry out the method step of salt-tolerant Sorbonne lily new line selection as follows:
[0040] a. Induction of lily embryogenic callus
[0041] Rinse the Sorbonne lily scales with running water for 1.5 hours; soak them in 75% alcohol for 30-40s, put them in 0.2% mercuric chloride for 7-9mins, rinse them with sterile water for 4-6 times, and then cut the scales into 0.5 Small pieces of cm*0.5cm are placed on the callus-inducing medium with the concave side facing up; the culture conditions are dark culture, the culture temperature is 23-26°C, and the culture time is 50-60 days, and the light yellow callus particles are induced to appear , standby; the callus induction medium is: MS+5mg / L2,4-D+6.5g / L agar+30g / L sucrose, the pH of the medium=5.8;
[0042] b. Configure salt-tolerant mutagenesis medium
[0043]Prepare the mutagenesis medium of MS+5mg / L2,4-D+2g / LNaCL+6.5g / L agar+30g / L sucrose in adv...
Embodiment 2
[0052] Example 2 Variety "wooden door (concad'or)"
[0053] Utilize EMS reagent mutagenesis to carry out the method step of the new line selection of salt-tolerant cayenne lily as follows:
[0054] a. Induction of lily embryogenic callus
[0055] Rinse the wooden door lily scales with running water for 0.5-2 hours; soak them in 75% alcohol for 30-40s, put them in 0.1%-0.2% mercuric chloride for 7-9mins, rinse them with sterile water for 4-6 times, and then put The scales were cut into small pieces of 0.5cm*0.5cm, and placed on the callus-inducing medium with the concave side facing up for cultivation; the cultivation conditions were dark cultivation, the cultivation temperature was 23-26°C, and the cultivation time was 50-60 days, and light yellow was induced The callus granules appear and are ready for use; the callus induction medium is: MS+5mg / L2,4-D+6.5g / L agar+30g / L sucrose, the pH of the medium is 5.9;
[0056] b. Configure salt-tolerant mutagenesis medium
[0057] Pr...
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Abstract
The invention discloses a method for breeding a salt-tolerant lily new strain by using EMS reagent mutagenesis. The method comprises the following steps of a, inducing lily embryonic calluses; b, preparing a salt-tolerant mutagenesis culture medium; c, performing salt-tolerant mutagenesis, namely placing the calluses on the salt-tolerant mutagenesis culture medium containing an EMS reagent for mutagenesis; d, performing inducing for seedling formation, namely sequentially inoculating the survival calluses onto a seedling formation culture medium and a bulb culture medium for culture, and growing lily bulblets with the diameter of 0.1 cm-0.5 cm for later use; and e, performing salt-tolerant screening, namely transferring the cultured lily bulblets with the diameter of 0.1 cm-0.5 cm to a screening culture medium for culture, wherein salt-tolerant lily bulbs which grow normally are the salt-tolerant lily new strain. According to the method, the method for breeding the salt-tolerant lily new strain through directional mutagenesis under the salt environment culture condition is easy to operate, high in operability and high in mutagenesis efficiency, and lily salt-tolerant germplasm resources can be greatly enriched within a short time.
Description
technical field [0001] The invention relates to the technical field of seedlings, in particular to a method for breeding new salt-tolerant lily strains by using EMS reagent mutagenesis, and the method can efficiently breed new salt-tolerant lily germplasm. Background technique [0002] With the expansion of lily production and bulb trade in the world, the production area of lily and the demand for bulbs are increasing. However, the growth conditions of lily are relatively strict, and they like to grow in a slightly acidic environment with good drainage, and are more sensitive to salt. Soil salinity is a key factor related to the normal growth of lilies. However, the water and soil in most parts of northern China are relatively saline and alkaline, which greatly limits the development of lily industry. [0003] At present, the research on salt-tolerant varieties of lily mainly focuses on the evaluation of salt-tolerant systems and field screening, but there are problems suc...
Claims
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Application Information
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