SCNN1 primer and diagnostic kit for clear cell nuclear cell carcinoma and application of SCNN1 primer

Abstract

The SCNN1 primer for rapidly diagnosing clear cell nuclear cell carcinoma provided by the invention comprises an internal reference primer pair which is GAPDH, mRNA sequence forward primers and reverse primers of SCNN1A genes with nucleotide sequences shown in SEQ ID NO: 1 and SEQ ID NO: 2, mRNA sequence forward primers and reverse primers of SCNN1B genes with nucleotide sequences shown in SEQ IDNO: 3 and SEQ ID NO: 4, and mRNA sequence forward primers and reverse primers of SCNN1G genes with nucleotide sequences shown in SEQ ID NO: 5 and SEQ ID NO: 6, which take human GAPDH as an internal reference control, and forward primers and reverse primers, having nucleotide sequences shown as SEQ ID NO: 7 and SEQ ID NO: 8. A corresponding kit is also prepared. The kit comprises the following reagents: a tissue lysis solution Trizol, an SYBRGreen Master Mix reaction solution, a reverse transcription reaction solution, a negative control and a positive control. The primer and the kit can eliminate false negative and false positive of clinical sample detection, and are safe, reliable, high in accuracy and suitable for clinical popularization.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a SCNN1 primer for renal clear cell carcinoma, a diagnostic kit and applications thereof. Background technique [0002] Renal cell carcinoma (RCC) is one of the most common malignant tumors in the urinary system, originating from renal tubular cells, among which clear cell nuclear cell carcinoma (ccRCC) accounts for 85% and ranks second in the malignant tumors of the urinary system. With the advancement of tumor detection methods and treatment methods, the survival rate of patients with clear cell renal cell carcinoma has been significantly improved, but the overall survival and progression-free survival are still low. Clear cell renal cell carcinoma is a tumor that develops relatively slowly, and its early symptoms often lack specificity. Many clear cell renal cell carcinomas are found in the middle and late stages, and more than 1 / 3 of the patients have metastas...

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research report on SCNN1 family in clear cell renal cell carcinoma, and the molecular mechanism of SCNN1 family in the development of clear cell renal cell carcinoma is still unclear

And there is no research report on the application of SCNN1 family in the preparation of renal clear cell carcinoma diagnosis or the prognosis of patients with renal clear cell carcinoma

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