Auratrilactone a, drug for treating anti-Alzheimer's disease, preparation method and application
A technology of acanthus trilactone A, which is applied in the field of medicine, can solve the problem of no activity of trilactone A compound of auranthus aurantium, and achieve the effect of preventing and treating the onset of Alzheimer's disease
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[0039] The present invention also provides the preparation method of beef tendon trihaldone A shown in the structure of the above scheme, including the following steps:
[0040] 1) Extraction of the beef band with 90% to 95% of the ethanol solution of 90% to 95%, collecting the extract; concentrates the extract, to obtain a dipper, resuspend the dipper in the water, resulting Rough liquid;
[0041] 2) EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc Ester extract;
[0042] 3) The ethyl acetate extract is subjected to a small hole resin chromatography, the first gradient elution, the first gradient elution process is: the volume fraction is 30% methanol, the volume fraction is 50%. The methanol aqueous solution, the volume fraction is 70% methanol aqueous solution, the volume fraction is 90% aqueous solution and anhydrous methanol for elution, collecting 5 parts of eluent, sequentially named FR1, FR2, FR3, FR4 and FR5;
[0043] 4) The FR4 performs a positive phase silica gel column chroma...
Embodiment 1
[0081]1) Buffali plant samples from the bullgle fruit small branch 100kg. The bullnut-branch dried pulverized into 80 to 100 mesh after the powder, the volume fraction was 95% ethanol aqueous solution, the extraction temperature was 60 ° C, and the amount of ethanol solution used at each extraction temperature was 100 L, and the heated reflux was extracted three times. Combined extract.
[0082] 2) The obtained extract is concentrated, and the solvent is recovered, the condensation temperature is 4 ° C, the pressure is 0.2 to 0.25 MPa, and the extract is obtained. The concentrated coarse strainer was subjected to 50L water suspension, and the extract was extracted three times in sequence, removing the petroleum ether extraction portion, collecting the residue, extracting the residue using 50 L of ethyl acetate to obtain a ethyl acetate extract .
[0083] 3) After obtaining ethyl acetate extract, the present invention performs a small pore resin (4 L) chromatographic separation, th...
Embodiment 2
[0114] The beef tendon trifle ester A, which is preceded by the method of Example 1, is added to the injection of water, fine filtration, and irrigation sterilization.
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