Harpertrioate A, medicine for treating and resisting Alzheimer's disease, preparation method and application
A technology of trilactone and trilactone, applied in the field of anti-Alzheimer's medicine and preparation, trilactone A, which can solve the problem of no activity of trilactone A compound question
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0039] The present invention also provides the preparation method of the auratrilactone A shown in the formula I structure in the above scheme, comprising the following steps:
[0040] 1) using an aqueous ethanol solution with a volume fraction of 90% to 95% to carry out reflux extraction on the branches of the tendon fruit, and collect the extract; concentrate the extract to obtain an extract; resuspend the extract in water to obtain crude extract;
[0041] 2) using petroleum ether to carry out the first extraction of the crude extract, remove the petroleum ether extraction part, and collect the residue; use ethyl acetate to carry out the second extraction of the residue, collect the ethyl acetate part, and obtain ethyl acetate ester extract;
[0042] 3) The ethyl acetate extract is subjected to small-pore resin chromatographic separation, and the first gradient elution is carried out. The procedure of the first gradient elution is: sequentially adopt methanol aqueous soluti...
Embodiment 1
[0081]1) The plant sample of beef tendon fruit is collected from 100 kg of beef tendon fruit sprigs. Drying and pulverizing the twigs of the beef tendon fruit into 80-100 mesh powders and then extracting by heating and refluxing with 95% aqueous ethanol solution by volume fraction, the extraction temperature is 60°C, the volume of the aqueous ethanol solution used for each extraction is 100L, and extracting by heating and refluxing three times, Combine the extracts.
[0082] 2) Concentrating the obtained extract under reduced pressure, recovering the solvent, the condensation temperature is 4° C., the pressure is 0.2-0.25 MPa, and the extract is obtained after concentration. Suspend and disperse the concentrated crude extract with 50L of water, extract three times with 50L petroleum ether, remove the petroleum ether extraction part, collect the residue, and extract the residue three times with 50L ethyl acetate to obtain ethyl acetate extract .
[0083] 3) After the ethyl ac...
Embodiment 2
[0114] The auratrilactone A prepared in advance by the method of Example 1 is added water for injection respectively according to the auxiliary agent used in conventional injections, finely filtered, potted and sterilized to make injections.
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size (mesh) | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More