Identification and diagnosis of multi-antigen protein combination applied to tuberculosis

A technology of antigenic protein and tuberculosis, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as time-consuming, high cost, and inability to meet rapid diagnosis

Active Publication Date: 2021-04-20
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of culturing mycobacteria in sputum takes a long time, usually 1-3 weeks or even 4-6 weeks, which cannot meet the needs of rapid diagnosis
Currently used molecular diagnostic techniques, such as the GeneXpert MTB/RIF method, although highly sensitive, can often detect the presence of Mycobacterium tuberculosis directly in fresh sputum or frozen sputum from patients within a relatively short period of time (about 2 hours) And resistance to rifampicin, but the whole process needs to be carried out in a closed environment, requires professional GeneXpert detection instruments and specimen collection boxes, and requires professionals to operate, the cost is high, and it is not conducive to large-

Method used

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  • Identification and diagnosis of multi-antigen protein combination applied to tuberculosis
  • Identification and diagnosis of multi-antigen protein combination applied to tuberculosis
  • Identification and diagnosis of multi-antigen protein combination applied to tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Take the second morning urine of the subject surnamed Wu, about 40ml of the middle urine from 7 to 11 am in a clean urine cup, transfer it to a sterile collection tube, and process it in the following way:

[0117] (1) The urine sample was immediately centrifuged at 1500g for 10 minutes in a centrifuge pre-cooled to 4°C in advance, and the supernatant was carefully transferred to a new sterile collection tube to remove the precipitated residue.

[0118] (2) Immediately add a protease inhibitor tablet (Roche, Germany, this tablet is suitable for sample collection with a volume of 40 ml) to each tube in this collection tube. After the inhibitor tablet is fully dissolved, freeze the collection tube immediately There is a -20°C freezer, avoid freeze-thaw during storage. The time from urine sample collection to final refrigerator storage was controlled within 4 hours.

[0119] (3) The collected urine samples were centrifuged at 4000 rpm for 20 minutes with a 50 kDa ultrafiltr...

Embodiment 2

[0142] Take the second morning urine of the subjects surnamed Ma, about 40 ml of mid-session urine from 7 to 11 am in a clean urine cup, transfer it to a sterile collection tube, and process it in the following way:

[0143] (1) The urine sample was immediately centrifuged at 1500g for 10 minutes in a centrifuge pre-cooled to 4°C in advance, and the supernatant was carefully transferred to a new sterile collection tube to remove the precipitated residue.

[0144] (2) Immediately add a protease inhibitor tablet (Roche, Germany, this tablet is suitable for sample collection with a volume of 40 ml) to each tube in this collection tube. After the inhibitor tablet is fully dissolved, freeze the collection tube immediately There is a -20°C freezer, avoid freeze-thaw during storage. The time from urine sample collection to final refrigerator storage was controlled within 4 hours.

[0145] (3) The collected urine samples were centrifuged at 4000 rpm for 20 minutes with a 50 kDa ultra...

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PUM

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Abstract

The invention relates to application of five antigen proteins in identification and diagnosis of active tuberculosis, provides a diagnostic reagent, and particularly relates to a novel tuberculosis diagnosis method which comprises the following steps of by taking the five antigen proteins P22352, Q9P121, P15151, Q13291 and Q8NDA2 as biomarkers, detecting the content of the five antigen proteins in urine, and taking the quantity value of the specific content as a diagnostic index, and indicating a negative result in the normal value range. And if the concentration of any three or more antigen proteins exceeds or is lower than the normal value range, the result is judged to be positive.

Description

technical field [0001] The invention relates to a diagnostic reagent, in particular to a multi-antigen protein applied to the identification and diagnosis of active tuberculosis. Background technique [0002] Tuberculosis is one of the major infectious diseases that endanger human health and is the main killer among adult infectious diseases in the world today, which has posed a serious challenge to international public health. my country is one of the countries with a high burden of tuberculosis in the world. The tuberculosis epidemic situation is "three highs and one low", that is, high prevalence, high mortality, high drug resistance rate, and low annual decline rate. With the co-infection of AIDS, drug-resistant tuberculosis and With the increase of floating population and other situations, the situation of prevention and control is very severe. Since the discovery of Mycobacterium tuberculosis, due to the characteristics of thick cell wall, high fatty acid content and i...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573
Inventor 金奇郑建华刘立国邓佳珩
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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