Molecular marker evaluation method for wild training panda immune adaptation

A technology of immune adaptation and molecular markers, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc., can solve the lack of training on giant panda immune characteristics, lack of monitoring means and evaluation methods, and the results of reactions Lag and other problems, to achieve the effect of enriching routine physical examination indicators, fast experimentation, and reliable results

Active Publication Date: 2021-04-30
CHINA CONSERVATION & RES CENT FOR THE GIANT PANDA SICHUAN
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of basic information on the immune characteristics of training giant pandas, the lack of monitoring methods and evaluation methods, it is impossible to evaluate and dynamically monitor the immune health of training giant pandas
Although giant pandas undergoing wild training also have regular physical examinations, routine physical examinations not only have few indicators, but also lag behind in response results, which cannot reflect the immune status of individuals in a timely, accurate and comprehensive manner. State and its dynamic changes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker evaluation method for wild training panda immune adaptation
  • Molecular marker evaluation method for wild training panda immune adaptation
  • Molecular marker evaluation method for wild training panda immune adaptation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A molecular marker evaluation method for the immune adaptation of wild trained giant pandas, using the innate and adaptive immune genes that are significantly differentially expressed between well-adapted wild trained giant pandas and captive giant pandas as markers, through real-time fluorescent quantitative PCR , to perform quantitative detection of the Marker in the sample to be tested.

Embodiment 2

[0046] This embodiment is on the basis of embodiment 1:

[0047] Special primers used in PCR amplification:

[0048] Marker1: forward primer 5'-GTGCAAGGGGACAGACGAAC-3'

[0049] Reverse primer 5'-CTTGAGTGTTTGCAGCCTTGGA-3';

[0050] Marker2: forward primer 5'-GATAGACCCACGACTGCACC-3'

[0051] Reverse primer 5'-GGTCTCGCCTCTTTCACAGG-3';

[0052]Marker3: forward primer 5'-CTGGCTGTTGCTCTCTTGGC-3'

[0053] Reverse primer 5'-ATGGTTTCTGTTTCTCAGCCCTCTT-3';

[0054] Marker4: Forward primer 5'-GAAGGTAAAAGTCGGGCCTGG-3'

[0055] Reverse primer 5'-GGCAGTTTTCTGCAGCTACTGTATG-3'

[0056] Marker5: forward primer 5'-CTACGACACGTCCACCGACA-3'

[0057] Reverse primer 5'-CCAAAGCCGCTGTTCTGGATG-3';

[0058] Marker6: Forward primer 5'-CGAGTCACCTACCAGGGCTA-3'

[0059] Reverse primer 5'-TCCGTGAACTCTGACTCCGT-3'.

Embodiment 3

[0061] This embodiment is on the basis of embodiment 1:

[0062] The primers of the internal reference gene amplified by real-time fluorescent quantitative PCR are:

[0063] Forward primer 5'-GGTATGACAACGAATTTGGCTACA-3'

[0064] Reverse primer 5'-GATGGAAACAGTGGAGGTCAGGA-3'.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a molecular marker evaluation method for wild training panda immune adaptation. The method comprises the following steps of: performing quantitative detection on Marker in a to-be-detected sample through real-time fluorescent quantitative PCR by using congenital immune and adaptive immune genes with remarkable differential expression between wild training pandas and captive pandas with good adaptation as Marker, and evaluating the immune adaptation condition of the wild training panda in the training period from the molecular biology level.

Description

technical field [0001] The invention relates to a method for evaluating the immune adaptation of giant pandas trained in the wild, in particular to a molecular marker evaluation method for the immune adaptation of wild trained giant pandas. Background technique [0002] The giant panda (Ailuropoda melanoleuca) is a rare and protected animal in China, and it is also the flagship species of biodiversity conservation in the world. As the time for captive giant pandas to be released into the wild gradually matures, rewilding training and reintroduction work has been put on the agenda. According to past experience, the survival rate of captive large mammals directly released into the wild is low. Reintroduced animals should undergo necessary wild training before release to restore their ability to adapt to the wild environment. The China Conservation and Research Center for the Giant Panda has officially started the wild training and reintroduction project of captive giant pand...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/124C12Q2600/156C12Q2561/113C12Q2563/107C12Q2545/114
Inventor 黄炎杨苗凌珊珊沈海波吴虹林何鸣张修月
Owner CHINA CONSERVATION & RES CENT FOR THE GIANT PANDA SICHUAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap