31results about How to "No sequencing required" patented technology

The invention relates to an authenticating method of fish hybrids and particularly relates to an RAG2 molecular marker RFLP identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, RAG2 gene sections in catfishes are selected to serve as molecular markers, the RFLP analysis of the RAG2 gene sections is achieved, and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, direct in identification and free from sequencing.

The invention relates to a group of specific primers and a method for identifying stiff silkworms, belonging to the technical field of identification of traditional Chinese medicines. According to themethod, after a stiff silkworm sample is crushed, the deoxyribonucleic acid (DNA) in domestic silkworms and the DNA in fungi are extracted at the same time by using a CTAB method; the extracted DNA is taken as a template, specific nucleotide sequences, which are designed and screened, are used as primers for performing polymerase chain reaction (PCR) amplification, and the products are then subjected to agarose gel electrophoresis analysis and gel imaging system detection, so that the authenticity and adulteration of the stiff silkworms can be finally detected based on the detection results.The method provided by the invention can be used for judging whether the sample is a stiff silkworm fake product or adulterated product, is simple to operate, does not needing sequencing, is high in specificity, rapid and accurate, and has unique advantages and important application value in the authenticity identification of stiff silkworm samples.

The invention relates to an improved primer for detecting the dynamic mutation of a CAG repetitive sequence of a spinocerebellar ataxia ATXN3 gene and a method thereof, characterized in that a specially designed specific primer is adopted to amplify the amount of a DNA fragment containing the CAG repetitive sequence in the ATXN3 gene to the observable degree through a special PCR procedure, and gel electrophoresis is utilized to observe the size of a PCR amplification product fragment and estimate the number of replication of the CAG dynamic mutation in the ATXN3 gene. The invention has the advantages that: 1. simplicity and economy, and no sequence detection, i.e. the method can be operated in common molecular biology laboratories or control laboratories, thereby establishing a simple detection method of the amplification CAG repetitive sequence; 2. high sensitivity and reliability: 25ng genome DNA is enough to detect the number of replication of the CAG dynamic mutation in the ATXN3gene, thereby a reliable basis is provided for clinically diagnosing spinocerebellar ataxia.