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A multiplex PCR detection kit for Anopheles claaus, Anopheles confused and Anopheles

A detection kit and technology for Anopheles, which can be used in multiplex PCR to simultaneously detect Anopheles claus, Anopheles cheetus, and Anopheles obfuscation, which can solve the problems of high cost, high requirements for personnel and equipment, and accurate results Difficult to guarantee and other issues, to achieve high specificity and sensitivity, low requirements for personnel and equipment, cost and time savings

Active Publication Date: 2022-06-07
JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the identification of Anopheles mosquitoes mainly relies on morphological identification, and the accuracy of the results is difficult to guarantee, and for incomplete samples and samples in the non-adult stage, morphological identification cannot be performed; the method of molecular markers is time-consuming and costly, and has a great impact on personnel and equipment. The requirements are also higher

Method used

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  • A multiplex PCR detection kit for Anopheles claaus, Anopheles confused and Anopheles
  • A multiplex PCR detection kit for Anopheles claaus, Anopheles confused and Anopheles
  • A multiplex PCR detection kit for Anopheles claaus, Anopheles confused and Anopheles

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Embodiment

[0032] 1. The Anopheles claunch primer screening test designed 6 groups of primers (numbered 1-6) according to the conserved sequence of the Anopheles cru mosquito gene (gene registration number MK685241.1):

[0033] Group 1:

[0034] F1：5’-ATGTAATTCCTGGAGATCGT-3’(SEQID NO.1)

[0035] R1：5’-GCTGGAGCATCAGTAGATT-3’(SEQID NO.2)

[0036] Group 2:

[0037] F2：5’-CTAATGTAATTCCTGGAGATCG-3’(SEQID NO.3)

[0038] R2：5’-GGAGCATCAGTAGATTTAGC-3’(SEQID NO.4)

[0039] Group 3:

[0040] F3：5’-TGTAATTCCTGGAGATCGTA-3’(SEQID NO.5)

[0041] R3：5’-TGCTGGAGCATCAGTAGA-3’(SEQID NO.6)

[0042]Group 4:

[0043] F4：5’-GCATGTGCTGTTACAATAAC-3’(SEQID NO.7)

[0044] R4：5’-GAGCTTGAGCCGGAATAG-3’(SEQID NO.8)

[0045] Group 5

[0046] F5：5’-ATCTACTGATGCTCCAGC-3’(SEQID NO.9)

[0047] R5：5’-ACCTCCTTCACTAACTCTT-3’(SEQID NO.10)

[0048] Group 6:

[0049] F6：5’-GCATGTGCTGTTACAATAAC-3’(SEQID NO.11)

[0050] R6：5’-GAGCTTGAGCCGGAATAGTA-3’(SEQID NO.12)

[0051] The PCR reaction solution used in primer screening is configure...

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Abstract

The invention relates to a kit for simultaneously detecting Anopheles claaus, Anopheles confused, and Anopheles aedes by multiplex PCR. The kit includes specific primers for An. clau, An. confused, and An. 2 , Taq enzyme, template DNA, sterile double-distilled water, the sequences of the specific primers of Anopheles claau, Anopheles confused, and Anopheles cheesa are respectively as SEQ ID NO.1-2, SEQ ID NO.15- 16, shown in SEQ ID NO.25-26. The multiplex PCR detection kit of the present invention can give qualitative detection results within 3 hours after receiving the DNA sample, saves cost and time, and has low requirements for personnel and equipment. An effective tool for Anopheles mosquitoes.

Description

Technical field [0001] The present invention relates to a multiple PCR simultaneous detection of Anopheles claudius, Anopheles mosquitoes, anopheles tabulaecal kit. Background [0002] Anopheles is a genus of Anopheles in the family Dipteroptera anopheles, known to be about 450 species and subspecies, divided into 6 subgenera, with a worldwide distribution, mainly distributed in the tropics. Anopheles females suck the blood of humans and animals, can spread malaria parasites, filariasis and other diseases, according to the World Health Organization, in 2016, 216 million cases of malaria occurred globally, 445,000 people died of malaria, the number of people threatened by lymphatic filariasis reached 893 million, and about 40 million people were disfigured by diseases and lost labor. [0003] With the change of natural conditions and the development of international transportation, trade and tourism, mosquitoes and their pathogens can spread internationally with the help of means ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/16C12Q2537/143C12Q2565/125Y02A50/30
Inventor 杨庆贵陶莉耿合员陈峰杨国平孙立新聂国嫒黄晓莉朱军胡双双谭伟龙贺骥
Owner JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE
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