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Fast method for detecting gene point mutation of receptor of epidermal growth factor lung cancer in non-cellule type

A technology of epidermal growth factor and non-small cells, applied in the field of biomedicine, can solve problems such as time-consuming, difficult to popularize and apply, and expensive equipment

Inactive Publication Date: 2007-02-07
SUZHOU KUANGYUAN MOLECULAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sequencing needs to measure the 18th, 19th, and 21st exon fragments of the epidermal growth factor receptor (EGFR), which needs to be carried out three times, which is not only time-consuming, but also expensive; This kind of instrument is very expensive, and general units cannot afford to equip it
Therefore, this method is difficult to apply clinically

Method used

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  • Fast method for detecting gene point mutation of receptor of epidermal growth factor lung cancer in non-cellule type
  • Fast method for detecting gene point mutation of receptor of epidermal growth factor lung cancer in non-cellule type
  • Fast method for detecting gene point mutation of receptor of epidermal growth factor lung cancer in non-cellule type

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Experimental program
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Embodiment

[0077] Embodiment: a method for rapidly detecting a point mutation of epidermal growth factor receptor gene in non-small cell lung cancer, comprising the following steps:

[0078] The first step, tumor tissue genomic DNA sample preparation

[0079] The first fresh or formalin-treated tissue:

[0080] Reagent preparation:

[0081] Buffer solution: 50mM Tris-HCl, pH 8.0

[0082] 20mM NaCl

[0083] 1 mM EDTA

[0084] 1% SDS

[0085] 10mg / ml proteinase K (Proteinase K): 10mg Proteinase K is dissolved in 1mlddH2O. Aliquot and store at -20°C. When in use, melt at 4°C. (It is best to use it now).

[0086] (1) Take a small amount of tissue (≤1 / 4 of the rice grain), add it to 20uL of the above solution (can be frozen), and then add 1uL of 10mg / ml proteinase K.

[0087] (2) Incubate at 55°C for 15 minutes, Vortex, and incubate for another 15 minutes. (PCR tubes can be used to complete on the PCR machine). Add double distilled water to 200uL.

[...

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Abstract

A method for quickly detecting the gene site mutation of epidermal growth fact receptor for non-small-cell lung cancer includes such steps as providing DNA, PCR amplifying by specially designed three groups of 4 primers, gel electrophoresis, and abserving result.

Description

technical field [0001] The invention relates to clinical detection technology in the field of biomedicine, in particular to a method for rapidly detecting the point mutation of epidermal growth factor receptor gene of non-small cell lung cancer. Using this gene detection method can not only quickly detect point mutations in certain regions of tumor tissue DNA of lung cancer patients, but more importantly, it can predict the new drug for the treatment of non-small cell lung cancer - Gefitinib (Trade name: Yirui) Sha (Iressa)] is effective for patients, guides clinical medication, and truly prescribes the right medicine to the case. Background technique [0002] Lung cancer is the tumor with the highest incidence rate in China, and the main pathogenic factor is smoking. According to medical statistics, 85% of male lung cancer and 75% of female lung cancer are caused by smoking. And other factors that can cause lung cancer include some exposure to industrial carcinogens, such...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王进朱辉秦正红
Owner SUZHOU KUANGYUAN MOLECULAR BIOTECH
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