Specific primers and method for identifying stiff silkworms

A specific and silkworm technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of inability to detect adulterated products, many steps, and time-consuming, etc., to achieve exclusive Strong, simple operation, no sequencing effect
CN107937567AActive Publication Date: 2018-04-20JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2018-04-20

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Abstract

The invention relates to a group of specific primers and a method for identifying stiff silkworms, belonging to the technical field of identification of traditional Chinese medicines. According to themethod, after a stiff silkworm sample is crushed, the deoxyribonucleic acid (DNA) in domestic silkworms and the DNA in fungi are extracted at the same time by using a CTAB method; the extracted DNA is taken as a template, specific nucleotide sequences, which are designed and screened, are used as primers for performing polymerase chain reaction (PCR) amplification, and the products are then subjected to agarose gel electrophoresis analysis and gel imaging system detection, so that the authenticity and adulteration of the stiff silkworms can be finally detected based on the detection results.The method provided by the invention can be used for judging whether the sample is a stiff silkworm fake product or adulterated product, is simple to operate, does not needing sequencing, is high in specificity, rapid and accurate, and has unique advantages and important application value in the authenticity identification of stiff silkworm samples.
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Description

technical field

[0001] The invention relates to a group of specific primers for identifying Bombyx mori and an identification method thereof, belonging to the field of identification of traditional Chinese medicines. Background technique

[0002] Bombyx mori is a dry body that is lethally infected (or artificially inoculated) with Beauveria bassiana (Bals.) Vuilknt by the 4th to 5th instar larvae of Bombyx mori Linnaeus. It is more than spring and autumn production, and the silkworms infected with Beauveria bassiana are dried. Silkworm was first recorded in "Shen Nong's Materia Medica", and has a long history of application. It is salty, pungent, flat, and belongs to the liver, lung, and stomach meridian. Modern pharmacological studies have shown that Bombyx mori has anticoagulant, anticonvulsant, anticancer, sedative and hypnotic effects.

[0003] Bombyx mori is a common traditional Chinese medicine for animals. It has reliable curative effect and high medicinal value. Th...

Claims

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