Internal transcribed spacer (ITS2) molecular marker identifying method for aquaculture of catfish hybrids

An aquaculture and molecular marker technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of low detection efficiency, affecting popularization and use, low repeatability, etc., and achieves easy operation, intuitive identification, and experimental quick effect

Inactive Publication Date: 2013-10-30
TONGWEI
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Problems solved by technology

However, the RAPD marker used in its identification work is limited by the method itself, and the detection efficiency is low and the repeatability is not high, which seriously affects the popularization and use of this method.

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  • Internal transcribed spacer (ITS2) molecular marker identifying method for aquaculture of catfish hybrids

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Embodiment Construction

[0023] The substantive content of the present invention will be further described in detail below in conjunction with specific embodiments.

[0024] ITS2 molecular marker identification method for aquaculture catfish hybrids, using southern catfish, catfish and their hybrid F 1 Generation as the research object, including the following steps:

[0025] The first step is to extract genomic DNA from southern catfish, catfish and their hybrid samples.

[0026] Because the individuals of the hybrid fry are small, the genomic DNA of the fry sample was extracted with the Marine Tissue DNA Extraction Kit (Tiangen Company).

[0027] Genomic DNA was extracted from parental southern catfish and catfish ray samples by conventional phenol-chloroform extraction:

[0028] a. Material collection: Take a small amount of fin ray sample soaked in absolute ethanol, cut it into pieces with alcohol-sterilized scissors, and put it into a 1.5 ml EP tube.

[0029] b Digestion: Add 500 μL lysis buff...

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Abstract

The invention relates to an ITS2 molecular marker identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, ITS2 gene sections in catfishes are selected to serve as molecular markers, the length difference the IST2 gene sections of the southern catfishes and the catfishes, and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.

Description

technical field [0001] The invention relates to a molecular marker identification method for fish hybrids, in particular to an ITS2 molecular marker identification method for aquaculture catfish hybrids. Background technique [0002] Siluriformes are carnivorous demersal fishes and are one of the most developed teleosts in fish evolution. Southern catfish (also known as big mouth catfish) ( Silurus meridionalis Chen) and catfish ( Silurus asotus Linnaeus) belong to Siluriformes, Siluridae, and Silurus genus, both of which are delicious and have high nutritional value, and are high-value-added economic fish mainly cultured in various parts of my country. Southern catfish is distributed in the Pearl River, Minjiang River, Xiangjiang River, Yangtze River and other water systems in my country. It is a large and ferocious carnivorous fish. It has the characteristics of fast growth, large body, and strong disease resistance. It likes to inhabit open water bodies, and The grav...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 刘匆郭睿宋昭彬盛晓洒应汝华陈齐勇孙家贤龚丽
Owner TONGWEI
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