Tissue culture and rapid propagation method of cynanchum glaucescens

A technology for tissue culture rapid propagation and staghorn fern, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of lack of large-scale reproduction and cultivation, and achieves the improvement of survival rate and optimization of tissue culture technology. Effect

Active Publication Date: 2021-06-04
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the staghorn ferns on the market come from direct imports. There is a lack of large-sc

Method used

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  • Tissue culture and rapid propagation method of cynanchum glaucescens
  • Tissue culture and rapid propagation method of cynanchum glaucescens
  • Tissue culture and rapid propagation method of cynanchum glaucescens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The establishment of embodiment 1 Chlorospora staghorn tissue culture rapid propagation method

[0046] 1 Materials and methods

[0047] 1.1 Test material

[0048] The spores of staghorn fern were collected from the greenhouse of Southwest Forestry University. Put the sporophyll in a sulfuric acid paper bag, collect the spores in a centrifuge tube after they scatter naturally, and store them in a seed cabinet at 4°C for later use.

[0049] 1.2 Test method

[0050] 1.2.1 Spore germination and sporophyte induction

[0051] The spores were sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with sterile water for 3 times, and inoculated on 1 / 2 MS medium. After the spores germinated until the dark green heart-shaped gametophytes appeared, they were transferred to MS, 1 / 2MS, 1 / 4MS, and improved Knops four kinds of media, agar 4g L -1 , pH 5.8. Inoculate 5 gametophytes per bottle, 5 bottles per treatment, and repeat 3 times. The growth of gametophytes a...

Embodiment 2 2

[0086] The optimization of embodiment 2 staghorn fern tissue culture rapid propagation technology

[0087] 1 Materials and methods

[0088] 1.1 Test material

[0089] The spores of staghorn fern were collected from the greenhouse of Southwest Forestry University. Put the sporophyll in a sulfuric acid paper bag, collect the spores in a centrifuge tube after they scatter naturally, and store them in a seed cabinet at 4°C for later use.

[0090] 1.2 Test method

[0091] 1.2.1 Spore germination and induction of GGB

[0092] The spores were sterilized with 5% sodium hypochlorite solution for 5 minutes, rinsed with sterile water for 5 times, and inoculated on 1 / 2 MS medium. After the spores germinated until dark green heart-shaped gametophytes appeared, they were subcultured until a small amount of sporophytes were induced. The sporozoites were transferred to the culture medium of 1 / 2MS+sucrose 15g / L+agar 4g / L, pH5.8. Add different types and concentrations of hormones, and dif...

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Abstract

The invention provides a tissue culture and rapid propagation method of cynanchum glaucescens. According to the tissue culture and rapid propagation method, spores of the cynanchum glaucescens are used as materials, tests and comparison are carried out in the aspects of spore germination, sporophyte induction, sporophyte root proliferation, rooting seedling treatment with allogenic materials to improve the adaptability of the sporophyte, a tissue culture and rapid propagation system of the neospora virens is established, the tissue culture process is optimized, the survival rate of transplanted and hardened seedlings is increased, and the mass breeding of the tissue culture seedlings of the cynanchum glaucescens is realized. According to the method, technical support is provided for industrial development and utilization of the cynanchum glaucescens, meanwhile, a guarantee is provided for population recovery and wild resource protection of the cynanchum glaucescens, the method is also suitable for tissue culture and rapid propagation of the chonemorpha bifida.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of staghorn fern (Platycerium). Background technique [0002] Platycerium wallichii Hooker (Platycerium wallichii Hooker) is a plant of the genus Platycerium in the Polypodiaceae family. It is a typical tropical rainforest epiphytic fern. There are about 15 species in this genus, which are distributed in Africa, tropical Asia and tropical America. In the rainforest. There is only one species in China, Chlorospora staghorn, which is distributed in the wild in Yingjiang County, Longchuan County and Ruili City, Dehong Prefecture, Yunnan Province. As a rare species of foliage plants, staghorn fern has a beautiful posture and has high ornamental value; as a symbol species of tropical rainforest, it can provide a basis for ecological restoration of tropical rainforest and has high ecological value; as a fern Evolutionary groups i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 向建英段杰秋唐军荣唐甜甜孙大宽邓国宾张媛
Owner SOUTHWEST FORESTRY UNIVERSITY
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