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A polypeptide combination marker, detection kit and method for early diagnosis of diabetes

A diagnostic kit and marker technology, applied in disease diagnosis, biological testing, measuring devices, etc., can solve the problems of low specificity, lack of sensitivity of markers, etc., and achieve high diagnostic specificity, high sensitivity, and good stability Effect

Active Publication Date: 2022-05-31
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of lack of markers for early warning and diagnosis of diabetes, low sensitivity and low specificity, and to provide a combination of polypeptide markers by using non-targeted peptidomics to discover differential polypeptides and targeted peptidomics verification. Its application and kit in early warning / diagnosis of diabetes, and also provides a method for the quantitative analysis and detection of the above-mentioned combined polypeptide markers

Method used

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  • A polypeptide combination marker, detection kit and method for early diagnosis of diabetes
  • A polypeptide combination marker, detection kit and method for early diagnosis of diabetes
  • A polypeptide combination marker, detection kit and method for early diagnosis of diabetes

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Effect test

Embodiment 1

[0044] The extraction of serum endogenous polypeptides: using ultrafiltration centrifugation. Specific operation process: Take blood from ‑80℃ refrigerator

[0047] Differential polypeptide screening: a total of 163 differential polypeptides were screened. Further select 21 in which the difference fold change is larger

[0049]

[0050]

Embodiment 2

[0054] The extraction of serum endogenous polypeptides: Reference Example 1.

[0071]

[0072]

[0073]

[0074] Note: L represents a light bid, and M represents a successful bid.

[0076] Stability evaluation: The stability of the target polypeptide was further evaluated, and the results showed that 5 polypeptides were placed in a 4°C refrigerator.

Embodiment 3

[0081] X=4.162+4.364×C(245)+8.905×C(244)-2.725×C(251)-3.326×C(248)-

[0082] Prob (diabetes)=1 / [1+e

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Abstract

The invention relates to the field of medical biological detection, in particular to the new application of polypeptide combination markers in the preparation of diabetes diagnostic reagents or kits. The present invention provides a method and kit for quantitatively detecting endogenous polypeptides in serum by using liquid chromatography-mass spectrometry multiple reaction monitoring (multiple reaction monitoring, MRM). Through the reagents and methods of the present invention, it is possible to quantitatively determine the endogenous polypeptides in human serum The content of the five endogenous polypeptide markers, and then calculate the combined marker variable and the determined cut-off value based on the binary logistic regression equation, for the early warning and / or diagnosis of diabetes. The kit and detection method of the invention have good stability and high specificity, and have the potential of clinical promotion.

Description

A peptide combination marker and detection kit for early diagnosis of diabetes and method technical field The present invention relates to the diagnosis, prevention and treatment field of medical biological detection technology and metabolic disease, specifically, It is based on the method for quantitative determination of human plasma endogenous polypeptides and the combination of polypeptide markers in the preparation of early warning and / or diagnosis of diabetes. Breakdown kits and methods. Background technique According to the data of International Diabetes Federation in 2017, there are 415 million diabetic patients in the world, of which type 2 diabetes (type 2 diabetes, T2DM) accounted for more than 95%. The number of people with diabetes worldwide is expected to increase to 552 million by 2030. Diabetes is the third major disease that endangers human health after cardiovascular disease and tumor. Currently, although the diagnosis of type 2 diabetes The s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/6848G01N2800/042G01N2800/52
Inventor 吴仁安王莉周孝禹
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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