Microbial mixed inoculant for promoting soybean growth and application thereof

A technology of mixing inoculants and microorganisms, applied in the field of agricultural microorganisms, can solve the problems of high production cost of chemical fertilizers, high cost of chemical fertilizers, poor quality of agricultural products, etc., and achieve the effect of enhancing the ability of crops to absorb water and fertilizer

Active Publication Date: 2021-06-29
兴安盟莱绅生物农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the main energy source for the production of nitrogen fertilizer was petroleum, but now it mainly relies on natural gas and coal, and these energy resources are in short supply. Because of the shortage,

Method used

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  • Microbial mixed inoculant for promoting soybean growth and application thereof
  • Microbial mixed inoculant for promoting soybean growth and application thereof
  • Microbial mixed inoculant for promoting soybean growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, Azospirillum brasilense (Azospirillum brasilense), Hydrogenophilus papieri Monoclonal Culture of (Hydrogenophaga palleronii)

[0041] The obtained Azospirillum brasilense (Azospirillum brasilense) was stained by Gram staining method, and the Azospirillum brasilense (Azospirillum brasilense) was Gram-negative and had a helical rod-shaped structure by optical microscopy. Colonies observed by naked eyes are characterized by round shape, low protrusions, round and complete edges, and light red color (see figure 1 and figure 2 ).

[0042] Take the nutrient gravy medium numbered (0002) of China Microorganism Culture Collection Center, take a beaker with a specification of 1L and dissolve the medium to 1L, adjust the pH of the medium to between 6.8 and 7.2 before high-temperature sterilization, and put the prepared 200ml Pour the culture medium into a 500ml Erlenmeyer flask, seal the high-temperature-resistant sealing film on the mouth of the Erlenmeyer fl...

Embodiment 2

[0045] Embodiment 2, the preparation of microbial mixed bacterial agent of the present invention

[0046] The fermented liquid containing Azospirillum brasiliensis cultivated in Example 1 and the fermented liquid containing Hydrogenophilus pareii are mixed in a volume ratio of 13:7 to form a microbial mixed bacterial agent (1L) (see Figure 5 ), the optimal concentration of Azospirillum brasiliensis was 1.9×10 8 cfu / ml; the optimal concentration of hydrogenophilic bacteria papierausus is 6.8×10 7 cfu / ml, the total bacterial concentration is not less than 1×10 8 cfu / mL.

[0047] Pour 200ml of improved AGAR medium (formulation as shown in Table 2) or BPY medium (formulation as shown in Table 3) into a 500ml Erlenmeyer flask, seal the high-temperature resistant sealing film on the mouth of the Erlenmeyer flask, and place it in a high temperature extinguisher. The bacteria are sterilized at 121°C for 30 minutes, and the above-mentioned microbial mixture can be added after coo...

Embodiment 3

[0052] Embodiment 3, plate counts the number of colonies of Azospirillum brasiliensis and Hydrogenophilus parethi in the microbial mixed bacterial agent of the present invention quantity

[0053] Get the microbial mixed bacterial agent that 1ml embodiment 2 prepares and carry out gradient dilution to 10 5 , and then take 25ul of the diluted bacterium solution and carry out coating (NA) nutrient agar plate, place it upside down and cultivate it at a biochemical constant temperature for 48h, and obtain the results as follows (see Figure 6 ):

[0054] The white dots are Azospirillum brasilense, and the black dots are Hydrogenophaga palleronii;

[0055] count to get Figure 6 There are 14 black punctuation points, Figure 6 continued There are 20 middle and black punctuation points, and the arithmetic mean is 17;

[0056] count to get Figure 6 There are 50 middle white punctuation points, Figure 6 continued There are 45 middle white punctuation points, and the arithme...

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Abstract

The invention relates to the technical field of agricultural microorganisms, and particualrly relates to a microbial mixed inoculant for promoting soybean growth and application thereof. The microbial mixed inoculant comprises fermentation liquor of azospirillum Brazii and hydrogenophilus paresis, wherein the concentration of the azospirillum Brazii is 1 * 10<6>cfu/ml-1 * 10<10>cfu/ml, and the concentration of the hydrogenophilus paresis is 1 * 10<6>cfu/ml-1 * 10<10> cfu/ml. The microbial mixed inoculant can stimulate plant cells to secrete various growth hormones, root increase, rooting, root strengthening and root capillarity are promoted powerfully, the length or width of the microbial mixed inoculant is increased, the water and fertilizer absorption capacity of crops is enhanced, the crops have the effects of being long and thick in stem, resistant to lodging and the like, then the yield of the crops is increased, and the problem that farmers abuse or use chemical fertilizer for a long time is solved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a microbial mixed bacterial agent for promoting soybean growth and its application. Background technique [0002] Soybean is one of the most important grains in my country. my country's soybean production ranks fourth in the world, and the soybean planting area increased in 2017 / 18. From the perspective of geographical division, my country's soybean production and planting areas mainly include four major regions, namely Heilongjiang planting area, Anhui planting area, Inner Mongolia Autonomous Region planting area and Jilin Province planting area, accounting for 36%, 11%, and 36% of the national planting area respectively. 8%, 4%. The average soybean yield in my country is 120 kg / mu, far below the international level of 200 kg / mu, and the domestic soybean yield needs to be improved. [0003] At present, soybean cultivation management mainly includes soybean v...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G22/40A01G7/06C12R1/01
CPCC12N1/20A01G22/40A01G7/06
Inventor 罗钰彬李华一金雁花杨效帆沈家葆
Owner 兴安盟莱绅生物农业有限公司
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