Pangolin coronavirus xCoV and application thereof and application of medicine in resisting coronavirus infection

A coronavirus and pangolin technology, applied in the direction of virus, virus/bacteriophage, microorganism-based methods, etc., to achieve high homology effect

Active Publication Date: 2021-06-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, due to the highly contagious nature of the virus, how to safely conduct drug screening and other related research and protect researchers from infection has become an urgent problem to be solved

Method used

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  • Pangolin coronavirus xCoV and application thereof and application of medicine in resisting coronavirus infection
  • Pangolin coronavirus xCoV and application thereof and application of medicine in resisting coronavirus infection
  • Pangolin coronavirus xCoV and application thereof and application of medicine in resisting coronavirus infection

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Embodiment Construction

[0045] The present invention is further described below in conjunction with embodiment. It should be noted that the following examples are not intended to limit the protection scope of the present invention, and any improvement made on the basis of the present invention does not violate the spirit of the present invention. The raw materials and equipment used in the present invention can be purchased commercially unless otherwise specified.

[0046] 1. Experimental method

[0047] 1. Cell Culture and Virus Culture

[0048] The African green monkey kidney cell line Vero E6 was obtained from the American Type Culture Collection (ATCC, No. 1586) at 37°C, 5% CO 2 Under the condition of , cultured in DMEM medium (Gibco) containing 10% fetal bovine serum (FBS; Gibco Invitrogen).

[0049] The pangolin isolate xCoV was propagated in Vero E6 cells, and the virus titer was determined by plaque assay. All infection experiments were performed in a biosafety level 2 (BLS2) laboratory. ...

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Abstract

The invention provides a coronavirus separated from pangolin. The coronavirus is named as pangolin coronavirus xCoV, the homology of the coronavirus xCoV and S protein of SARS-COV-2 reaches 92.5%, receptors of xCoV infected cells and the SARS-COV-2 are consistent and are angiotensin converting enzyme 2 (ACE2). However, the xCoV does not infect people, is very safe to people, can be used for screening active drugs and vaccines for resisting the SARS-COV-2 virus, and can also be used for preparing attenuated vaccines or inactivated vaccines for resisting the SARS-COV-2 virus. Based on the pangolin coronavirus xCoV, a plurality of active compounds with anti-coronavirus activity are obtained by screening, EC50, CC50 and SI evaluation is performed on cepharanthine (stephanine), silamectin and mefloquine hydrochloride (mefloquine) in the active compounds, and the xCoV inhibition mechanism of cepharanthine is analyzed and researched through transcriptome sequencing.

Description

technical field [0001] The invention belongs to the field of medicines, and specifically relates to a pangolin coronavirus xCoV and its application for screening anti-SARS-COV-2 virus drugs, including a drug screening model and a drug screening method of the pangolin coronavirus xCoV, and also relates to a drug screening method based on the pangolin coronavirus xCoV. The active compound screened by xCoV is used for the preparation of a medicine for treating SARS-COV-2 viral infectious diseases. Background technique [0002] The new coronavirus (named "SARS-COV-2" by the World Health Organization, previously named 2019 new coronavirus or 2019nCoV) belongs to the genus βcoronavirus, has an envelope, and the particles are round or oval, often polymorphic Sex, diameter 60-140nm. Its genetic characteristics are significantly different from those of SARS-CoV and MERS-CoV. Current research shows that it has more than 85% homology with bat SARS-like coronavirus (bat-SL-CoVZC45). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/70C12R1/93
CPCC12N7/00C12Q1/701C12N2770/20021C12N2770/20034C12Q2600/136Y02A50/30
Inventor 童贻刚范华昊宋立华安小平王立钦刘文丽刘振东
Owner BEIJING UNIV OF CHEM TECH
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