A kind of swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and its preparation method and application

A technology of porcine pseudorabies virus and porcine circovirus, applied in chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of prone to spread, economic loss of pig farming, and sick pigs Severe symptoms and other problems, to achieve the effect of reducing the number of vaccinations, reducing labor costs, and increasing protein production

Active Publication Date: 2022-07-12
WUHAN KEQIAN BIOLOGY CO LTD
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, clinically, mixed infection of swine fever, porcine pseudorabies and porcine circovirus disease is relatively common. Once mixed infection occurs, the symptoms of sick pigs will be more serious, the mortality rate will be higher, and it is prone to diffuse transmission. Pig farming causes serious economic losses

Method used

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  • A kind of swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and its preparation method and application
  • A kind of swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and its preparation method and application
  • A kind of swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Construction of recombinant CHO cell lines expressing swine fever E2 protein, porcine pseudorabies virus gB protein and gD protein respectively

[0038] 1. Gene synthesis

[0039] 1.1 Synthesis of CSFV E2 gene

[0040] CSFV E2 protein is the main protective antigen of CSFV and can induce specific immune responses in animals. In this protocol, the C-terminal transmembrane region of the E2 protein was removed, and the corresponding codon optimization was carried out in order to be more suitable for expression in CHO cells. The IL-2 signal peptide sequence was added to the N-terminus of the optimized E2 sequence, with His tags on both ends. Synthesized CSFV-E2 fragment, see sequence pattern diagram figure 1 , see SEQ ID NO: 1 for the sequence. The synthesized fragment was placed on the plasmid vector pUC-18 (Invitrogen), which was obtained in the form of pUC-E2.

[0041] 1.2 Synthesis of PRV gB gene

[0042] The full length of gB is 914 amino acids. In this scheme,...

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Abstract

The invention relates to a swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and a preparation method and application thereof. The immunogenic components in the triple subunit vaccine include swine fever virus E2 protein, porcine pseudorabies virus gB and gD proteins, and porcine circovirus type 2 Cap protein. The present invention significantly improves the expression level of swine fever virus E2 protein, porcine pseudorabies virus gB and gD protein by various optimizations, and the immunogenicity after expression, and is matched with porcine circovirus. The type 2 Cap protein was prepared to obtain a triple immunization vaccine, and the immunization effect against swine fever virus, porcine pseudorabies virus and porcine circovirus was significantly improved.

Description

technical field [0001] The invention relates to the field of veterinary biotechnology, in particular to a swine fever, porcine pseudorabies and porcine circovirus type 2 triple subunit vaccine and a preparation method and application thereof. Background technique [0002] Swine fever is an acute and highly contagious infectious disease, and the outbreak of CSF severely restricts the healthy development of the pig industry. At present, CSF still occurs continuously in pig farms in many regions, and the epidemic form is mainly sporadic, with atypical clinical symptoms. Rabbit-attenuated swine fever vaccine has good immunogenicity and safety, and plays an important role in the prevention and control of swine fever. The eradication and purification of the plague. Therefore, it is necessary to develop a marker vaccine to quickly and easily distinguish vaccine-immunized animals from wild virus-infected animals, thereby providing an effective monitoring method for the eradication...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/187A61K39/12A61K39/245A61P31/20A61P31/14A61P31/22C12N15/85C12N15/866C12N5/10
CPCA61K39/12A61P31/20A61P31/14A61P31/22C12N15/85C12N15/86C07K14/005C12N2770/24334C12N2710/16734C12N2750/10034A61K2039/70A61K2039/552C12N2800/107C12N2800/105C12N2710/14043C12N2770/24322C12N2770/24351C12N2710/16722C12N2710/16751C12N2750/10022C12N2750/10051
Inventor 周明光张华伟徐高原郝根喜曾小燕安春敬周飞燕汤细彪朱娴静
Owner WUHAN KEQIAN BIOLOGY CO LTD
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