Electrophoresis control method and equipment

A control method and electrophoresis technology, which are applied in the direction of material analysis, measuring devices, and instruments by electromagnetic means, can solve the problems of less acquisition, waste of resources, influence experiments, etc., and achieve faster separation speed, increased migration distance, The effect of increased migration speed

Pending Publication Date: 2021-07-13
SHANGHAI TANON LIFE SCI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When it is difficult to obtain biomacromolecule samples and the amount obtained is very small, it will seriously affect the experiment and waste resources

Method used

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  • Electrophoresis control method and equipment
  • Electrophoresis control method and equipment
  • Electrophoresis control method and equipment

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Embodiment Construction

[0035] The present invention will be further described now in conjunction with accompanying drawing.

[0036] The current various electrophoresis (horizontal electrophoresis, vertical electrophoresis and disc electrophoresis) are positive and negative electrodes through the buffer, so that the entire gel forms a single DC electric field.

[0037] However, in the present invention, the electric field of electrophoresis is divided into several segmental electric fields E1, E2 to En along the material separation direction in the gel. see figure 1 , figure 1 Shown is a schematic diagram of an embodiment of the method of the present invention, wherein the magnitude, direction, or action time of at least one section of the segmented electric field is set to be independently controllable, that is, each group of electrode connections can be independently controlled for voltage, current, timing, and activation. The start time and end time and the DC power supply for positive and nega...

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Abstract

An electrophoresis control method and equipment are disclosed, an electrophoresis electric field is divided into a plurality of segmented electric fields, and the electrophoresis electric field is divided into a plurality of segmented electric fields in the material separation direction; the size, the direction and the action time of at least one segment of the segmented electric field are set to be independently controlled; and the material separation speed, direction and part are adjusted by changing the size, direction and action time of the segmented electric field. According to the invention, a plurality of segmented electric fields of which the sizes and directions can be independently controlled are divided in the material separation direction, so that the speed, the moving direction and the part of a material during electrophoresis are controlled.

Description

technical field [0001] The invention relates to the field of electrophoresis, in particular to an electrophoresis control method and equipment. Background technique [0002] The electrophoresis tank is often used in biomedical biomacromolecule separation experiments. A kind of gel, a cathode electrode in the electrophoresis tank, a buffer solution with conductive properties for immersing the cathode end of the gel and the cathode electrode of the electrophoresis tank, and for the anode end of the immersion gel and the anode electrode of the electrophoresis tank form a direct current circuit loop. [0003] The mixture of biological macromolecules (DNA or RNA or protein) with different molecular weights in negative ion state after treatment is powered by the DC electric field of the electrophoresis tank, and migrates from the cathode end of the electrophoresis tank to the anode end in the gel with molecular sieve function. And because of the principle that the larger the mole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44704
Inventor 孟诚卫
Owner SHANGHAI TANON LIFE SCI CO LTD
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