Method for screening garlic fructan stress response key enzyme genes and metabolites based on transcriptome and metabolome

Abstract

The invention discloses a method for screening garlic fructan stress response key enzyme genes and metabolites based on a transcriptome and a metabolome. Through a transcriptome and metabolome conjoint analysis method, fructan-related differential expression genes measured by a transcriptome and fructan-related substances with obvious metabolic change measured by a metabolome are subjected to conjoint analysis, a gene-metabolite network interaction diagram can be constructed, and the relationship between the genes and the metabolites can be better analyzed.

Description

technical field [0001] The invention relates to the technical field of multi-omics joint analysis, in particular to a method for screening key enzyme genes and metabolites of garlic fructan stress response based on transcriptome and metabolome. Background technique [0002] Fructan is a water-soluble oligomeric or polysaccharide, a non-cellular carbohydrate composed of multiple fructose residues connected to a single sucrose end in the form of glycosidic bonds. As an important osmotic regulator, fructan's metabolism is closely related to plant stress resistance. Studies have found that the synthesis and catabolism of fructan in plants is an important protection mechanism for plants to adapt to adverse environmental factors, and its accumulation can enhance the stress resistance of plants under various environmental stresses (Xu Huanhuan et al., 2014). There are three main reasons why fructan improves plant stress resistance (Sun Yongmei et al., 2015): (1) Through the accumu...

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Problems solved by technology

[0004] In the prior art, there is no screening technology for garlic fructan stress response-related genes and metabolites, and there are few reports on the key genes and metabolites of garlic fructan metabolism, so its metabolic mechanism in garlic is still unclear