RNA (Ribonucleic Acid) interference fragment of donkey Zfy gene, expression vector and application of RNA interference fragment

A technology of RNA interference and expression vectors, applied in the field of molecular genetics, can solve problems such as difficult control, large randomness, and instability, and achieve low-cost effects

Pending Publication Date: 2021-07-16
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the factors that determine the sex of animals are mainly the internal factors of the animal body. The method of controlling the external environment to control the sex of birth animals is random, unstable, and difficult to control.

Method used

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  • RNA (Ribonucleic Acid) interference fragment of donkey Zfy gene, expression vector and application of RNA interference fragment
  • RNA (Ribonucleic Acid) interference fragment of donkey Zfy gene, expression vector and application of RNA interference fragment
  • RNA (Ribonucleic Acid) interference fragment of donkey Zfy gene, expression vector and application of RNA interference fragment

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Embodiment Construction

[0015] The technical solutions provided by the present invention will be described in detail below in conjunction with the examples, but they should not be interpreted as limiting the protection scope of the present invention.

[0016] 1. Construction of the RNA interference fragment and expression vector of the donkey Zfy gene

[0017] This application has screened 5 pairs of siRNA fragments through preliminary work, and then according to the requirements of the vector and the promoter, add restriction sites, loops and termination sequences to the 5' and 3' of each siRNA fragment, and the final form The shRNA oligonucleotide sequence is: T+sense strand 19nt target sequence+stem-loop structure (TTCAAGAGA)+target sequence reverse complement sequence+RNAPolyIII polymerase transcription termination site (TTTTTT)+XhoI restriction site (GAGCT) ( Entrust Shanghai Biological Engineering Co., Ltd. to synthesize the shRNA oligonucleotide sequence), as shown in Table 1.

[0018] Table ...

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Abstract

The invention belongs to the technical field of molecular genetics, and provides an RNA (Ribonucleic Acid) interference fragment of a donkey Zfy gene, an expression vector and application of the RNA interference fragment. The RNA interference fragment provided by the invention comprises a sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The RNA interference fragment provided by the invention is used for artificially interfering the expression of the donkey Zfy gene so as to block generation and development of Y sperms, thereby creating more fertilization opportunities for X sperms to increase the proportion of female donkeys of first filial generation; and thus, the purpose of controlling the genders of offspring donkeys is achieved.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to an RNA interference fragment of a donkey Zfy gene, an expression vector and applications thereof. Background technique [0002] It is of great significance to realize the sex control of livestock. The significance of animal sex control lies in: (1) improving the production efficiency of animal husbandry; (2) producing animals of the sex with utilization value (generally, the utilization value of female animals is higher than that of male animals); Twin infertility caused by embryos; (4) increase the intensity of selection; (5) prevent the occurrence of sex-linked genetic diseases, etc. Research on sex control of animals has started many years ago. Animal sex control (sex control) refers to a biotechnology that makes adult female animals produce offspring of the desired gender by artificially intervening in the normal reproductive process of animals. At present, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/85C12N15/12C12N5/10
CPCC07K14/47C12N15/113C12N15/85C12N2310/14
Inventor 贾斌孙玉江郑新宝肖海霞李梦雨张志东营瑞文李超程申红蒋松
Owner SHIHEZI UNIVERSITY
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