Mutation characteristic-based determination method for small cell lung cancer molecular typing and application

A technology for small cell lung cancer and molecular typing, applied in the field of molecular typing of small cell lung cancer based on mutation characteristics

Pending Publication Date: 2021-09-10
AFFILIATED CANCER HOSPITAL OF SHANDONG FIRST MEDICAL UNIV SHANDONG CANCER INST (SHANDONG CANCER HOSPITAL)
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Problems solved by technology

[0005] The difficulty in solving the above problems and defects is: since TP53 and RB1 are the two most common mutations in small cell lung cancer, it is difficult to carry out molecular typing again based on the characteristics of gene changes

Method used

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  • Mutation characteristic-based determination method for small cell lung cancer molecular typing and application
  • Mutation characteristic-based determination method for small cell lung cancer molecular typing and application
  • Mutation characteristic-based determination method for small cell lung cancer molecular typing and application

Examples

Experimental program
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Embodiment 1

[0074] DNA library construction and whole exome sequencing (WES).

[0075] Genomic DNase was enzymatically cut into 200bp fragments. T-joints were added at both ends after end repair and A-tailing. For library construction, purified DNA was amplified by ligation-mediated PCR, followed by generation of the final sequencing library using 96RXN xGen Exome Research Panel v1.0 (Integrated DNA Technologies, USA) according to the manufacturer's instructions. Paired-end multiplexed samples were sequenced using the NovaSeq6000 system. The sequencing depth was 200× for each tissue sample and 100× for each control.

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Abstract

The invention belongs to the technical field of genes, and discloses a mutation characteristic-based determination method for small cell lung cancer molecular typing and an application. The method comprises the following steps: analyzing gene change characteristics of small cell lung cancer, performing signature correlation analysis on a genome, performing unsupervised clustering, and determining a typing result of small cell lung cancer molecules. Through a method of whole exon sequencing (WES), characteristics of gene mutation, copy number change and the like of 178 cases of small cell lung cancer are comprehensively analyzed, and the immune characteristics of TMB, TNB, PDL1, CD8 + T cells and the like are analyzed. According to the invention, gene characteristics and immune characteristics of the small cell lung cancer are integrated. Three gene change molecular types of the small cell lung cancer are provided for the first time. The method is essential for understanding the characteristics of heterogeneity of the small cell lung cancer and developing individualized treatment, and can promote the development of individualized clinical experiments of the small cell lung cancer.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to a method and application for determining molecular typing of small cell lung cancer based on mutation characteristics. Background technique [0002] At present, small cell lung cancer (SCLC) has a high degree of malignancy and poor prognosis. Chemotherapy or chemotherapy combined with radiotherapy has been used clinically as two important treatment methods. Recently, although immunotherapy has made some research progress in small cell lung cancer, however, the progress is limited. Due to the unique biological characteristics of small cell lung cancer, it is of great clinical significance to determine and analyze the characteristics of its genome. In fact, molecular biology studies have shown that small cell lung cancer can be divided into four subtypes, SCLC-A, SCLC-N, SCLC-P, and SCLC-Y, and these subtypes can provide necessary clues for personalized treatment. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/68G16B30/00G16B50/10
CPCC12Q1/6886G01N33/57423G01N33/57492G01N33/68G16B30/00G16B50/10C12Q2600/156C12Q2600/112G01N2333/70532G01N2333/70517
Inventor 王海永王哲海赵成龙林家茂李振祥孙健李成于海宁
Owner AFFILIATED CANCER HOSPITAL OF SHANDONG FIRST MEDICAL UNIV SHANDONG CANCER INST (SHANDONG CANCER HOSPITAL)
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