The invention relates to a time-resolved
fluorescence immunochromatography test strip for simultaneously detecting
influenza A virus, influenza B
virus and novel
coronavirus antigens in a
throat swaband a preparation method thereof. A sample pad, an
influenza A virus, influenza B virus and novel
coronavirus antibody labeled time-resolved fluorescent
microsphere combination pad, a reaction film and a water absorption pad are sequentially adhered to a PVC (
polyvinyl chloride) bottom plate in a staggered manner by about 2mm, and
then test strips are fixed by an upper plastic clamping shell and alower plastic clamping shell which are matched with each other to form the
test card. The reaction film is pre-coated with an
influenza A virus
capture antibody (T1 detection line), an influenza B virus
capture antibody (T2 detection line), a novel
coronavirus capture antibody (T3 detection line) and a goat anti-mouse polyclonal
antibody (C
control line). A time-resolved
fluorescence immunochromatography technology is introduced into detection of
pharyngeal swab influenza A virus,
pharyngeal swab influenza B virus and novel coronavirus, so that the detection time is greatly saved, the detection stability and sensitivity are improved, the operation is simple and convenient, and the kit can be used for
field detection; and meanwhile, the time-resolved
fluorescence immunochromatography teststrip has the advantages of low cost and high
cost performance.