52results about How to "Convenient clinical diagnosis" patented technology

The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.

The invention relates to a one-tube magnetic bead method for extraction of virus nucleic acid for fluorescent quantitative detection, which belongs to the technical field of biological nucleic acid application and includes: firstly, preparing lysis solution, adding the lysis solution and magnetic beads into a tube strip, adding a sample of virus nucleic acid into the tube strip, and mixing well to lead the magnetic beads to fully absorb the virus nucleic acid; secondly, placing magnetic bead mixed liquor on a magnetic carrier, and allowing the liquor to stand for seconds to separate the magnetic beads and solution; and thirdly, rinsing for protein removal and desalination, and drying in air at room temperature. The method is simple and quick, less apt to cause pollution and high in flexibility. By the method, extracting efficiency is high, nucleic acid purity is high, testing repeatability is good, compatibility to amplification reagents is high, production cost is reduced, operation accuracy is improved, and works such as clinical diagnosis, medicolegal identification and the like are facilitated greatly.

The invention discloses a metal artifact correction method for multi-energy spectrum X-ray CT imaging. Firstly, the multi-energy spectrum X-ray CT projection image containing the metal artifact is subjected to weighted image reconstruction and material decomposition to obtain a weighted reconstructed image I0, a projection image of a metal material and other base materials; then, the metal material image Im is reconstructed according to the projection image of the metal material as a priori knowledge in the subsequent iterative reconstruction process; a virtual monoenergetic projection patternPs is generated according to the projection patterns of all the decomposed base materials and their X-ray attenuation coefficients at a given energy; finally, using I0 as the initial image, Im as theprior knowledge of iterative image reconstruction and Ps as the target projection in the iterative process, the final metal artifact correction CT image is obtained by using the statistical iterativereconstruction algorithm.

The invention discloses a method and a system for controlling X-ray doses of a mammary machine. The method includes: subjecting targeted mammary glands different in nature to pre-exposure to obtain pre-exposure images and subjecting the pre-exposure images to analytical calculation to obtain the X-ray doses of the targeted mammary glands at different projection angles to ensure acquirement of the best shot images. Due to the fact that an X-ray dose value of each projection angle is best set and the final X-ray dose values are lowered, X-ray radiation effect upon a patient is effectively reduced, image quality is improved, clinical diagnosis is benefited, and doses values of X-ray radiation that the patient received are lowered.

The invention relates to a time-resolved fluorescent immunochromatographic test strip for quantitatively detecting CA153 in blood and a preparation method thereof. The test strip is a test card formedby successively bonding an NC film, a CA153 antibody and mouse anti-rabbit IgG marked fluorescent microsphere conjugate pad, a sample pad and an absorbent pad on a white PVC substrate staggeredly at2 mm and then using upper and lower matched plastic card holder to fix the test strip for assembling, and the NC film is pre-coated with a CA153 antibody detection line and a rabbit IgG quality control line. According to the test strip, a time-resolved fluorescence immunochromatography technology is introduced into the quantitative detection of the CA153 in the blood, the detection time is greatlysaved, the stability and sensitivity of detection are improved, the operation is simple and convenient, and the test strip can be used for on-site screening; the test strip has the advantages of lowcost and high cost performance.

The invention discloses a method for constructing a DNA library and application of the method. The method for constructing the DNA library comprises the steps: providing DNA of a chimeric marker, wherein the DNA of the chimeric marker has three-dimensional structure information; carrying out transposition treatment on the DNA of the chimeric marker to obtain a transposition product; carrying out capture treatment on the transposition product to obtain captured DNA; and carrying out amplification treatment on the captured DNA so as to obtain the DNA library. The library construction method is simple in steps, short in consumed time, particularly suitable for library construction of trace DNA samples, high in effective data proportion of sequencing and low in noise single-end suspension value.

A digital 12-channel electrocardiograph able to record standard 12 human electrocardiac signals in 12 channels, output the irritation signals for regulating esophageal pulse, and record esophageal electrocardiac signal for diagnosing cardiovascular diseases is composed of leads combinant circuit, cardioelectric amplifier, microprocessor, printer, display unit, sound-light alarm unit, keyboard, memory, communication interface LAN, real-time clock circuit, peripheral interfaces and power supply.

Methods and systems are provided for whole-body spine labeling. In one embodiment, a method comprises acquiring a non-functional image volume of a spine, acquiring a functional image volume of the spine, automatically labeling the non-functional image volume with spine labels, automatically correcting the geometric misalignments and registering the functional image volume, and propagating the spine labels to the functional image volume. In this way, the anatomical details of non-functional imaging volumes may be leveraged to improve clinical diagnoses based on functional imaging, such as diffusion weighted imaging (DWI).

The invention provides a fluorescent quantitative PCR detection kit for mitochondrion deafness A7445G mutation. The kit is prepared from quantitative PCR reaction liquid, a first positive control substance, a second positive control substance, a negative control substance, a specification and a box body, wherein the quantitative PCR reaction liquid is prepared from a PCR buffer solution, MgCl2, dNTPs, a heat-resistant DNA polymerase, an upstream amplification primer, a downstream amplification primer, a first fluorescent probe and a second fluorescent probe. According to the kit provided by the invention, two MGB probes are designed for the A being larger than G single-base mutation on the site 7445 of mtDNA; the nucleotide on the site 7445 of the deafness related mitochondrion is detected through fluorescent quantitative PCR to determine whether the A being larger than G mutation happens on the site; the kit is simple, quick, accurate and the like and can be applied to clinical diagnosis and control of mitochondrion A7445G mutation related deafness and also provides a basis for the prevention and treatment of mitochondrion deafness caused by A7445G mutation.

The invention relates to a time-resolved fluorescence immunochromatography test strip for simultaneously detecting influenza A virus, influenza B virus and novel coronavirus antigens in a throat swaband a preparation method thereof. A sample pad, an influenza A virus, influenza B virus and novel coronavirus antibody labeled time-resolved fluorescent microsphere combination pad, a reaction film and a water absorption pad are sequentially adhered to a PVC (polyvinyl chloride) bottom plate in a staggered manner by about 2mm, and then test strips are fixed by an upper plastic clamping shell and alower plastic clamping shell which are matched with each other to form the test card. The reaction film is pre-coated with an influenza A virus capture antibody (T1 detection line), an influenza B virus capture antibody (T2 detection line), a novel coronavirus capture antibody (T3 detection line) and a goat anti-mouse polyclonal antibody (C control line). A time-resolved fluorescence immunochromatography technology is introduced into detection of pharyngeal swab influenza A virus, pharyngeal swab influenza B virus and novel coronavirus, so that the detection time is greatly saved, the detection stability and sensitivity are improved, the operation is simple and convenient, and the kit can be used for field detection; and meanwhile, the time-resolved fluorescence immunochromatography teststrip has the advantages of low cost and high cost performance.

The invention provides an intracardiac multi-mode ultrasonic imaging method, device and system, and the method comprises the steps: enabling an ultrasonic imaging catheter to be inserted into a cardiac cavity along a blood vessel, and carrying out the real-time B-mode imaging of the interior of the cardiac cavity; adjusting the position of the probe; setting an acquisition period of multi-modal ultrasonic signal acquisition, and adjusting the position information to perform multi-modal ultrasonic signal acquisition on the plurality of to-be-observed areas; and performing data processing on the ultrasonic signals under each piece of position information, and obtaining the cardiac cavity tissue characteristics of each to-be-observed area in the acquisition period in combination with a time sequence. According to the scheme, four-dimensional multi-mode imaging and parameter measurement can be carried out on the heart tissue, related information such as a three-dimensional heart cavity structure, myocardial tissue displacement, strain and elasticity at different moments of a heartbeat cycle is measured from the interior of a heart cavity, the heart cavity tissue characteristics of the heart tissue are obtained, and multi-dimensional mechanical parameter measurement is achieved.

The invention relates to the image processing field and discloses an ultrasonic image de-noising and enhancement algorithm. The ultrasonic image de-noising and enhancement method comprises steps of (1) using Curvelet transformation to perform decomposition on an image to obtain a low frequency sub-band and high frequency sub-band of different sizes and different directions, (2) multiplying a low frequency sub-band coefficient with a small gain coefficient and inhibiting image background information, (3) adopting a high frequency gain function having a direction selection to process a Curvelet high frequency sub-band coefficient, configuring a big gain multiple and a small threshold for the high frequency sub-band close to a horizontal direction, configuring a small gain multiple and a big threshold for the high frequency sub-band close to a perpendicular direction, and (4) performing Curvelet inverse transformation on the processed coefficient to obtain an enhanced image. The ultrasonic image de-noising and enhancement method can effectively remove spot noise of the ultrasonic image and can enhance edge information in the image.

The invention discloses a combined detection test strip for heavy metal and creatinine, and a preparation method and an application thereof. The invention aims to provide a rapid, accurate, simple, and inexpensive test strip for simultaneously detecting the heavy metal ions and the creatinine. The technical points of the invention is that: the detection test strip comprises a bottom plate, which is sequentially connected with a sample pad, a bonding pad, a chromatographic membrane, a creatinine test paper, and an absorbent pad. The chromatographic membrane is provided with a C-line and a T-line. The bonding pad is sprayed with a heavy metal ion antibody labeled with the same colloidal gold particle. The chromatographic membrane is coated with a heavy metal ion antigen labeled with fluorescent microspheres at T-line position and a BSA labeled with the fluorescent microspheres at the C-line position. The invention relates to the field of immunochromatography detection of fluorescence anddry chemical analysis.

The present disclosure is directed to multiplex assays, kits and methods, including automated methods, for identifying PD-L1 positive immune cells, PD-L1 positive tumor cells, and PD-L1 negative immune cells within a tissue sample using two dyes selected from diaminobenzidine (DAB), 4-(dimethylamino) azobenzene-4′-sulfonamide (DABSYL), tetramethylrhodamine (DISCOVERY Purple), N,N′-biscarboxypentyl-5,5′-disulfonato-indo-dicarbocyanine (Cy5), and Rhodamine 110 (Rhodamine).

The invention discloses a system for automatically analyzing a dopamine transporter PET image based on a CT structure image. The system comprises an image acquisition module, an image preprocessing module, a DAT-PET functional image automatic segmentation module and a bilateral striatum sub-partition automatic numerical value extraction and calculation module. And based on the CT structure image, fine automatic segmentation of a striatum region and striatum sub-partitions (including front and rear shell cores on both sides and front and rear caudal cores on both sides) in the DAT-PET function image is carried out. The invention comprises performing fine automatic segmentation and three-dimensional reconstruction display on striatum of a DAT-PET functional image by a synchronously scanned CT structural image, and automatically calculating developer uptake values and uptake value ratios of striatum sub-partitions. Clinical doctors can be effectively assisted in interpreting the DAT-PET functional image, a reliable basis is provided for diagnosis and differential diagnosis of Parkinson's syndrome, and the invention has high robustness.

A model training method and an electronic device are provided. The method includes the following steps: establishing a brain age prediction model according to a training set; adjusting a parameter in the brain age prediction model according to a validation set; inputting a test set into the brain age prediction model with the adjusted parameter to obtain a plurality of first predicted brain ages; determining whether the first predicted brain ages satisfy a first specific condition; and completing training of the brain age prediction model when the first predicted brain ages satisfy the first specific condition.

The invention relates to a multifunctional chair for a hospital. The multifunctional chair comprises a chair body; a base plate is arranged below one chair supporting leg of the chair body; a support is vertically connected to the base plate; a supporting plate capable of vertically sliding along the support is arranged in the middle of the support; and a hook is arranged at the top of the support. The support is a rod body with the height larger than 1.5 m, and the bottom end of the support is fixedly connected to the base plate. When the blood pressure is measured, as the level zero position of a sphygmomanometer, the elbow of the upper right arm of a person and the heart of the human body should be kept in one plane, the multifunctional chair is provided with the support and the supporting plate, and the position of the supporting plate can be vertically adjusted according to needs; the sphygmomanometer is placed on the supporting plate, the position of the supporting plate is adjusted by the seat height of the patient, and then blood pressure measurement is carried out; and positioning can be achieved rapidly and accurately, and accuracy of measurement results is guaranteed. Meanwhile, as the hook is arranged at the top of the support, if the patient needs to be infused, an infusion bottle can be hung on the hook, and the chair has the multiple functions.

The invention discloses a structured clinical diagnosis term set construction method and system. The method comprises the following steps of constructing a term set hierarchical structure in advance;importing multiple pieces of term set basic information and determining a distribution position of each piece of term set basic information in the term set hierarchical structure; determining a mapping relation between different term set basic information based on the distribution positions of the different term set basic information in the term set hierarchical structure according to a preset matching rule and/or relevance of the term set basic information in the existing clinical diagnosis information; and storing the imported term set basic information according to the respective distribution position in the term set hierarchical structure and the mutual mapping relation. According to the method and the system, the clinical diagnosis of a doctor is facilitated, and a hospital can carryout fine management on the diagnosis information.

The invention discloses a nuclear magnetic resonance imaging enhancement method based on dictionary learning, a computer device and a storage medium, and the method comprises the steps: obtaining original scanning data, an original image and a dictionary, executing a first iteration process for many times, and the like, wherein each first iteration process performs image reconstruction processing according to an optimization target, the optimization target is determined by the original scanning data and a sparse vector in the corresponding first iteration process, and the sparse vector is determined by the dictionary and a to-be-processed image in the corresponding first iteration process. According to the method, the original scanning data and the original image acquired by the nuclear magnetic resonance equipment are acquired, multi-round iteration is performed on the original scanning data and the original image to perform dictionary learning, image enhancement can be performed on the basis of an image processing result of the nuclear magnetic resonance equipment, and the image quality is further improved; therefore, clinical diagnosis and other researches are facilitated. The method is widely applied to the technical field of image processing.

The invention relates to a fetal electrocardiogram accurate extraction method based on ant colony constraint blind source separation. A demixing matrix W is solved by utilizing ant colony optimization, the demixing matrix can be optimized in real time according to constraint conditions, then a fetal electrocardiogram signal can be effectively and accurately extracted, and further the quality of a fetus signal is improved, so that clinical diagnosis can be benefited.