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Test strip for detecting influenza viruses and preparation method thereof

A type of influenza B virus and detection area technology, applied in the field of medical testing, can solve problems such as the inability to guarantee the quality of test units, poor sensitivity and repeatability, and affect test results, so as to facilitate clinical diagnosis, improve stability and sensitivity , the effect of easy operation

Inactive Publication Date: 2019-04-05
SHENZHEN YILIFANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Each test unit of POCT is independent, so the quality of each test unit cannot be guaranteed to be the same
Based on immunochromatographic test strips and instruments, the test results will be affected by the difference in temperature, humidity and pH value
The methodological flaws of some POCT instruments, such as poor sensitivity and repeatability, and narrow linear range, are only used for reference in emergencies or emergencies, and need to be sent to the laboratory for re-examination if necessary.

Method used

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  • Test strip for detecting influenza viruses and preparation method thereof
  • Test strip for detecting influenza viruses and preparation method thereof
  • Test strip for detecting influenza viruses and preparation method thereof

Examples

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preparation example Construction

[0044] A preparation method for preparing the time-resolved immunochromatographic test strip for detecting influenza A / B virus, comprising the following steps:

[0045] (1) On the reaction membrane 3, respectively immobilize the influenza A / B influenza monoclonal capture antibody and the goat anti-mouse IgG antibody recognizing a single epitope to form a detection area and a quality control area;

[0046] (2) Prepare time-resolved fluorescent microsphere-labeled influenza A / B influenza virus monoclonal detection antibody, and spray on the binding pad 2;

[0047] (3) Paste the sample pad 1, the binding pad 2, the reaction membrane 3 and the water-absorbing pad 4 on the bottom plate 5 in order to obtain the time-resolved fluorescent immunochromatographic test strip.

[0048] In a preferred embodiment, step (2) further includes the following steps:

[0049] (2.1) Use 0.01-0.05mol / L MES activation buffer of pH 5.5-6.0 to wash the microspheres, add carbodiimide (EDC) and N-hydroxy...

Embodiment 1

[0072] Example 1 Time-resolved immunochromatographic test strips for detecting influenza A / B viruses.

[0073] A time-resolved immunochromatographic test strip for detecting influenza A / B virus of the present embodiment, comprising a PVC bottom plate, and a sample pad, a binding pad, a reaction membrane and a water-absorbing pad sequentially arranged on the PVC bottom plate, The binding pad is coated with influenza A / B monoclonal detection antibody labeled with fluorescent microspheres, and the reaction membrane includes a detection area and a quality control area arranged in parallel and separated by 6 mm from each other, and the detection area is close to the The binding pad, the quality control area is close to the water-absorbing pad, the detection area is coated with a monoclonal capture antibody for influenza A / B that recognizes a single antigenic epitope, and the quality control area is coated with goat antibody Mouse IgG antibody.

[0074] In this embodiment, the reac...

Embodiment 2

[0083] Example 2 A time-resolved immunochromatographic kit for detecting influenza A / B viruses.

[0084] The time-resolved fluorescence immunochromatography kit for detecting influenza A / B virus of this embodiment, said kit includes: the test strip described in Embodiment 1, a plastic cartridge, and a sample buffer.

[0085] In this embodiment, the sample buffer contains 0.5% BSA, 0.05% Tween-20 in PBS pH7.4 buffer solution.

[0086] When the time-resolved fluorescent immunochromatography kit for detecting influenza A / B virus of the present invention is used, the throat swab sample to be tested is added to the sample buffer, and 50 μL of sample dilution is added dropwise on the sample pads of A and B respectively. liquid, which transports the sample to the conjugate pad by capillary action. When the throat swab sample contains influenza A / B virus, it forms an antigen-antibody complex with the monoclonal antibody on the fluorescent microspheres. With the action of chromatograp...

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Abstract

The invention discloses a time resolution immunochromatographic test strip for detecting influenza A / B viruses and a preparation method thereof. The test strip comprises a bottom board and a sample pad, a combination pad, a reaction membrane and a water absorption pas, which are pasted on the bottom board in sequence, wherein the combination pad is coated with an influenza A / B virus monoclonal detection antibody marked by a time resolution fluorescent microsphere; the reaction membrane comprises a detection area and a quality control area parallel to and spaced from the detection area; the detection area is close to the combination pad; the quality control area is close to the water absorption pad; and the detection area and the quality control area are respectively coated with an influenza A / B viruses monoclonal capture antibody and a goat anti mouse IgG antibody for recognizing single epitopes. The time resolution immunochromatographic test strip for detecting influenza A / B viruses is high in sensitivity, simple to operate, low in cost and short in detection time, and is capable of effectively satisfying the demand of rapid clinical examination.

Description

technical field [0001] The invention relates to medical testing technology, in particular to a time-resolved fluorescent immunochromatographic test strip for detecting influenza virus and a preparation method thereof. Background technique [0002] Influenza (abbreviated as influenza) is an acute respiratory infection caused by influenza virus, and it is also a disease with strong contagion and fast transmission speed. It is mainly spread through droplets in the air, person-to-person contact or contact with contaminated items. Typical clinical symptoms are: sudden onset of high fever, general pain, significant fatigue and mild respiratory symptoms. Generally, autumn and winter are the high-incidence period, and the complications and death caused by it are very serious. The disease is caused by influenza virus and can be divided into three types: A, B, and C. Among them, the influenza epidemic caused by Type A is the most widespread and severe, Type B often causes outbreaks,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/558G01N33/569G01N33/577G01N21/64
CPCG01N21/6408G01N21/6428G01N33/533G01N33/558G01N33/56983G01N33/577
Inventor 廖生赟胡涛陈建东张贵
Owner SHENZHEN YILIFANG BIOTECH CO LTD
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