Microsatellite marker of homatula fish as well as amplification primer and application of microsatellite marker

A microsatellite marker and microsatellite technology, applied in biochemical equipment and methods, microbe measurement/inspection, climate change adaptation, etc.

Active Publication Date: 2021-09-10
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In recent years, the research on the genus Homeria mainly focused on the interspecific or intraspecific morphological differences and the taxonomic status of the genus and species, but there were few studies on its genetic diversity and genetic structure. The research based on the markers developed by nuclear genes little research

Method used

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  • Microsatellite marker of homatula fish as well as amplification primer and application of microsatellite marker
  • Microsatellite marker of homatula fish as well as amplification primer and application of microsatellite marker
  • Microsatellite marker of homatula fish as well as amplification primer and application of microsatellite marker

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Experimental program
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Embodiment 1

[0042] 1. Search for sequences containing microsatellite repeat units

[0043] Use the software SSRHunter1.3 to search for the sequence of microsatellite repeat units from the sequences of the constructed gene libraries of the three species of fish of the genus Homer. more than one sequence. A total of 192 sequences containing microsatellite repeat units were screened out, and primers were designed from them for polymorphism detection. Among them, the three species of the Homer genus were red-tailed Homer, short-bodied Homer and shellfish. Homer's loach.

[0044] 2. Design of microsatellite primers:

[0045] From the gene sequences containing microsatellite repeat units, select the sequences that meet the primer design and use PrimerPremier 5.0 to design primers. The main parameters are set as follows: the primer length is 17-25bp, 20bp is the optimum length, the PCR product fragment length range is 100-350bp, and the optimum annealing temperature is 55-65°C. The GC conten...

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Abstract

The invention discloses a microsatellite marker of homatula fish as well as an amplification primer and application of the microsatellite marker. According to the microsatellite marker, 17 microsatellite markers are screened from genomic DNAs of three types of fishes (homatula red-tail, homatula brachysome and homatula bainite) of homatula, specific primers are designed in flanking areas at two ends of a microsatellite repetitive sequence for amplification, the obtained amplification product has high polymorphism and good stability, the microsatellite marker can be used in the fields of population genetic diversity, genetic structure, genetic resource protection and the like of three types of fishes of the homatula.

Description

technical field [0001] The invention belongs to the technical field of DNA markers in molecular biology, and in particular relates to a microsatellite marker for fish of the genus Homeria genus and its amplification primer and application. Background technique [0002] Homatula belongs to Cypriniformes, Cobitoidea, and Nemacheilidae. It is an endemic group in China. It has 12 species and is widely distributed in the Yangtze River, Yellow River, Pearl River and Lancang River systems. . In recent years, the research on the genus Homeria mainly focused on the interspecific or intraspecific morphological differences and the taxonomic status of the genus and species, but there were few studies on its genetic diversity and genetic structure. The research based on the markers developed by nuclear genes Research is almost nonexistent. Collected from three species of Homer loach distributed in the middle and upper reaches of the Yangtze River, the red-tailed Homer loach, the short-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2525/151Y02A40/81
Inventor 熊美华邵科徐念曾昌朱滨李伟涛阙延福
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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