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Culture medium for primary umbilical cord mesenchymal stem cells and application of culture medium

A mesenchymal stem cell and culture medium technology, which is applied in the field of culture medium of primary umbilical cord mesenchymal stem cells, can solve the problem that the culture medium is not stable, and achieve the effect of shortening the preparation time and improving the cell viability

Active Publication Date: 2021-09-17
廊坊康宝汇泰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the preparation method of primary stem cells, some patents improve the traditional tissue block attachment method by optimizing the culture medium, such as patents CN201480030461. Used in the subculture process, although it also improves the isolation culture cycle, but the self-produced medium is not as stable as the commercialized medium

Method used

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  • Culture medium for primary umbilical cord mesenchymal stem cells and application of culture medium
  • Culture medium for primary umbilical cord mesenchymal stem cells and application of culture medium
  • Culture medium for primary umbilical cord mesenchymal stem cells and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Umbilical cord mesenchymal stem cell supernatant and its primary umbilical cord mesenchymal stem cell culture medium

[0040] 1. Preparation of umbilical cord mesenchymal stem cell supernatant:

[0041] (1) Collect umbilical cord mesenchymal stem cell culture fluid, add citrate fatty acid glyceride, the content of described citrate fatty acid glyceride is 0.03%, mix;

[0042] (2) Add guayulelide to the mixture obtained in step (1), the concentration of guayulelide is 100 μg / mL, mix well, and heat at 37° C. for 10 minutes;

[0043] (3) The mixture in step (2) was centrifuged at 4°C and 12000 r / min for 10 min, and the supernatant was taken.

[0044] 2. Primary umbilical cord mesenchymal stem cell culture medium

[0045] Including the umbilical cord mesenchymal stem cell supernatant prepared in step 1, the serum-free medium of mesenchymal stem cells (Gibco company, product number C12571500BT or AventaCell company, product number HPCFDCRL50) and guayulelide (Sig...

Embodiment 2

[0046]Example 2 Umbilical cord mesenchymal stem cell supernatant and its primary umbilical cord mesenchymal stem cell culture medium

[0047] 1. Preparation of umbilical cord mesenchymal stem cell supernatant:

[0048] The umbilical cord mesenchymal stem cell culture fluid was collected, centrifuged at 12000 r / min at 4°C for 10 min, and the supernatant was taken.

[0049] 2. Primary umbilical cord mesenchymal stem cell culture medium

[0050] Including the umbilical cord mesenchymal stem cell supernatant, mesenchymal stem cell serum-free medium and guayulelide prepared in step 1, wherein the volume of the umbilical cord mesenchymal stem cell supernatant and mesenchymal stem cell serum-free medium The ratio was 1:1, and the final concentration of guayulelide was 10 μmol / L.

Embodiment 3

[0051] Example 3 Umbilical cord mesenchymal stem cell supernatant and its primary umbilical cord mesenchymal stem cell culture medium

[0052] 1. Preparation of umbilical cord mesenchymal stem cell supernatant:

[0053] (1) Collect umbilical cord mesenchymal stem cell culture fluid, add citrate fatty acid glyceride, the content of described citrate fatty acid glyceride is 0.03%, mix;

[0054] (2) Add guayulelide to the mixture obtained in step (1), the concentration of guayulelide is 100 μg / mL, mix well, and heat at 37° C. for 10 minutes;

[0055] (3) The mixture in step (2) was centrifuged at 4°C and 12000 r / min for 10 min, and the supernatant was taken.

[0056] 2. Primary umbilical cord mesenchymal stem cell culture medium

[0057] Including the umbilical cord mesenchymal stem cell supernatant and the mesenchymal stem cell serum-free medium prepared in step 1, wherein the volume ratio of the umbilical cord mesenchymal stem cell supernatant to the mesenchymal stem cell ser...

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Abstract

The invention belongs to the technical field of cell culture, and particularly relates to a culture medium for primary umbilical cord mesenchymal stem cells and application of the culture medium. The umbilical cord mesenchymal stem cell supernate and / or parthenolide are / is used for culturing the primary cells, compared with a traditional tissue block adherence method, the preparation time of the primary umbilical cord mesenchymal stem cells can be effectively shortened, and good cell viability, phenotype and function of the primary umbilical cord mesenchymal stem cells are kept.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a medium for primary umbilical cord mesenchymal stem cells and an application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells derived from the mesoderm in the early stage of development. In bone marrow, fat, umbilical cord, placenta, teeth, synovium and other tissues. Among them, umbilical cord mesenchymal stem cells (Umbilicalcord Mesenchymal Stem Cells, UCMSCs) refer to a kind of pluripotent stem cells existing in the umbilical cord tissue of newborns. Ethical controversy, low immunogenicity and other advantages. [0003] Umbilical cord mesenchymal stem cells have the ability to differentiate into adipocytes, chondrocytes and bone cells under specific induction conditions in vivo or in vitro. Because of its multi-directional differentiation potential, it can be widely used in the treatment of nervous ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/90C12N2500/36C12N2500/30C12N2502/1388
Inventor 成芳苏晓华
Owner 廊坊康宝汇泰生物技术有限公司
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