A reagent and method for transporting cells
A cell and reagent technology, applied in the field of cell biology, can solve the problems of time-consuming, laborious, costly and expensive cell transportation, and achieve the effects of low cost, maintaining viability and recovery ability, and convenient configuration
Active Publication Date: 2022-05-31
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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Problems solved by technology
[0008] In order to solve the time-consuming, laborious and costly problems of cell transportation in the prior art, the present invention provides a reagent for cell transportation and a method for cell transportation
Method used
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Effect test
Embodiment
[0039] 8 ℃-16 ℃: express in Chongqing urban area, 2-3 months ambient temperature 8 ℃-16 ℃;
[0041] 37°C: constant temperature shaker at 37°C, low speed 10 times / day, 2 minutes each time.
[0044] Conclusion: cell viability is closely related to ambient temperature and transport time. Under the same conditions, the temperature is 2℃‑8℃ and 8
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[0047] *, p<0.05, **, p<0.01, compared with the same row 2 ℃-8 ℃
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[0051] *, p<0.05, **, p<0.01, compared with the same row 2 ℃-8 ℃
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[0055] *, p<0.05, **, p<0.01, compared with the same row 2 ℃-8 ℃
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The invention discloses a reagent and method for transporting cells. The reagent is composed of 50%-100% serum, 0%-50% cell culture medium, penicillin and streptomycin, and is prepared by using the reagent after digestion of cells cultured in vitro The cell suspension can maintain more than 80% cell viability during long-distance transportation at an ambient temperature of 2-30 degrees.
Description
A kind of reagent and method for transporting cells technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a reagent and method for transporting cells. technical background In vitro cell culture refers to simulating the physiological environment in the body in vitro, in aseptic, appropriate temperature, pH and certain A method by which mammalian cells grow, reproduce, and maintain their structure and function under nutrient conditions. Cell culture technology Widely used in biological and medical research fields. Cells often need to be transported from one laboratory to another to meet the needs of the world Various research needs in the world, the culture conditions, ambient temperature, transportation time, etc. of the cells in the process of cell transportation It has a great influence on the physiological state of cells. At present, in order to ensure the activity of cells during transportation, cells...
Claims
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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 毕杨周红孙艳婷张楠楠
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV