Eight metabolites as biomarkers for sperm impairment

Abstract

The invention discloses eight metabolites as biological markers for sperm damage, and a method comprises the following steps: (1) experiment grouping: dividing a sample into three parts: A, semen of a normal sperm donation voluntary; B, semen of a patient with varicocele before an operation; C, post-operation semen of a varicocele patient; (2) experiment steps: A, performing denoising or screening operation on metabolite qualitative and (relative) quantitative results, and when denoising or screening operation needs to be performed on the metabolite qualitative and (relative) quantitative results, performing name duplication material screening on the metabolite qualitative and (relative) quantitative results, and then performing noise elimination on the metabolite qualitative and (relative) quantitative results. By adopting the detection method designed by the invention, the newly found eight metabolites have the prediction efficiency that AUC is greater than 90%, whether sperms are damaged or not and the quality of the sperms can be judged by utilizing the eight metabolites existing in the sperms, and great convenience is brought to the detection work of the quality of the sperms.

Description

technical field [0001] The invention relates to the technical field of infertility, and specifically eight kinds of metabolites are used as biological markers of sperm damage. Background technique [0002] In the early stage, we used metabolomics technology to screen out 8 metabolite small molecules from the comparison of 30 samples of normal people, patients with varicocele and patients with improved semen quality after varicocele surgery. The single or combined detection of these metabolites can effectively diagnose the sperm damage markers of patients with varicocele, and can be used for the diagnosis of varicocele and the discrimination of whether there is a surgical pointer. Using these 8 metabolites in semen, can Determination of sperm damage and a measure of semen quality. Contents of the invention [0003] The purpose of the present invention is to provide 8 kinds of metabolites as biological markers of sperm damage, which have the advantages of rapid detection an...

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