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High-stability bone and bone tumor tissue microarray chip and preparation method thereof

A tissue microarray technology with high stability, which is applied in the preparation of test samples, measuring devices, and material analysis through optical means, and can solve problems such as denaturation, less cells, and effective target tissues that are dropped from the chip, and achieve improved The effect of core quality, saving time and cost, and improving the level of scientific research

Active Publication Date: 2021-11-02
中科光华西安智能生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems existing in the prior art, the present invention provides a high-stability bone and bone tumor tissue microarray chip and its preparation method. Denaturation and other issues, saving time and cost, improving the core quality of tissue chips

Method used

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  • High-stability bone and bone tumor tissue microarray chip and preparation method thereof
  • High-stability bone and bone tumor tissue microarray chip and preparation method thereof
  • High-stability bone and bone tumor tissue microarray chip and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0063] A method for preparing a highly stable bone and bone tumor tissue microarray chip of the present embodiment comprises the following steps:

[0064] (1) collecting bone and bone tumor tissue samples, and processing the tissue samples;

[0065] The specific processing of the tissue samples is as follows: fix the bone and bone tumor samples in a formalin solution with a volume fraction of 10% for 24 hours, then saw the bone slices or bone tumor tissues with a thickness of 0.5 cm, and then The fraction is fixed in 10% formalin solution for 48 hours, bone or tumor tissue with bone is placed in decalcification agent, concentrated hydrochloric acid 15ml, sodium chloride 7.5ml, distilled water are added to 100ml to obtain described decalcification agent , a new decalcifying agent needs to be replaced every day until the bone tissue is easily penetrated by the needle, then dehydrated, transparent, dipped in wax, and sliced;

[0066] (2) Embedding the treated tissue samples in p...

Embodiment 2

[0070] A method for preparing a highly stable bone and bone tumor tissue microarray chip of the present embodiment comprises the following steps:

[0071] (1) collecting bone and bone tumor tissue samples, and processing the tissue samples;

[0072] The specific processing of the tissue samples is as follows: fix the bone and bone tumor samples in a formalin solution with a volume fraction of 10% for 24 hours, then saw the bone slices or bone tumor tissues with a thickness of 0.5 cm, and then The fraction is fixed in 10% formalin solution for 48 hours, bone or tumor tissue with bone is placed in decalcification agent, concentrated hydrochloric acid 15ml, sodium chloride 7.5ml, distilled water are added to 100ml to obtain described decalcification agent , a new decalcifying agent needs to be replaced every day until the bone tissue is easily penetrated by the needle, then dehydrated, transparent, dipped in wax, and sliced;

[0073] (2) Embedding the treated tissue samples in p...

Embodiment 3

[0077] A method for preparing a highly stable bone and bone tumor tissue microarray chip of the present embodiment comprises the following steps:

[0078] (1) collecting bone and bone tumor tissue samples, and processing the tissue samples;

[0079]The specific processing of the tissue samples is as follows: fix the bone and bone tumor samples in a formalin solution with a volume fraction of 10% for 24 hours, then saw the bone slices or bone tumor tissues with a thickness of 0.5 cm, and then The fraction is fixed in 10% formalin solution for 48 hours, bone or tumor tissue with bone is placed in decalcification agent, concentrated hydrochloric acid 15ml, sodium chloride 7.5ml, distilled water are added to 100ml to obtain described decalcification agent , a new decalcifying agent needs to be replaced every day until the bone tissue is easily penetrated by the needle, then dehydrated, transparent, dipped in wax, and sliced;

[0080] (2) Embedding the treated tissue samples in pa...

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Abstract

The invention relates to a high-stability bone and bone tumor tissue microarray chip and a preparation method thereof. The bone and bone tumor tissue microarray chip prepared by the method disclosed by the invention well solves the problems of chip point dropping, less effective target tissues, cell degeneration and the like. From the perspective of physics, plane arrangement and practicability, the optimal bone tumor tissue chip arrangement mode is comprehensively designed; according to the scheme, the time and the cost are saved, the core quality of the tissue chip is improved, and the tissue chip is well received by vast scientific research, clinical and teaching institutions in the fields of antibody research and development, tumor research, gene detection and the like. The chip basically meets the requirements of a control group sample required by research and development of targeted drugs and tumor diagnosis and generation mechanism and outcome in tumor biological treatment in the fields of tissues, proteins, gene molecules and the like, so that the scientific research level is more quickly, conveniently, comprehensively and rigorously improved.

Description

technical field [0001] The invention belongs to the technical field of tissue chips, and in particular relates to a highly stable bone and bone tumor tissue microarray chip and a preparation method thereof. Background technique [0002] Tissue chip, also known as tissue microarray, is an important branch of biochip technology. It arranges many different individual tissue samples on the same carrier (mostly glass slides) in a regular array, and is mainly used to study the same gene or The expression of protein molecules in different cells or tissues. Since the technology came out in 1998, it has been popularized and applied in a wide range due to its large-scale, high-throughput, and standardization advantages. Its biggest advantage is that the experimental conditions of the tissue samples on the chip are completely consistent and have excellent quality control. Saving time and saving reagents is obvious. [0003] my country's tissue chip preparation technology is becoming...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/36G01N1/31G01N21/84
CPCG01N1/28G01N1/36G01N1/31G01N21/84
Inventor 陈鑫杨江涛刘蓓
Owner 中科光华西安智能生物科技有限公司
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