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Composite microcarrier for manufacturing cell culture meat and preparation method of composite microcarrier

A microcarrier and cell technology, applied in microcarriers, biochemical equipment and methods, cell culture supports/coatings, etc., can solve the problems of myoblast differentiation carriers that have not yet been published, and achieve material cost and production cost Reduced, high biocompatibility effect

Pending Publication Date: 2021-11-23
CHINA MEAT RES CENT
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cell culture can be used for large-scale cell production through fermenters, cell factories, etc., but there are few reports on cell differentiation and myogenic research.
In particular, the research on the differentiation direction of myoblasts has not yet published the emergence of vectors that can effectively regulate and promote the differentiation of myoblasts

Method used

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  • Composite microcarrier for manufacturing cell culture meat and preparation method of composite microcarrier
  • Composite microcarrier for manufacturing cell culture meat and preparation method of composite microcarrier
  • Composite microcarrier for manufacturing cell culture meat and preparation method of composite microcarrier

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Embodiment Construction

[0025] Attached below Figure 1-7 The present invention is described in detail:

[0026] The present invention is used for the composite microcarrier of cell cultivation meat and mainly comprises microcarrier sphere, and microcarrier sphere is an irregular sphere, and the surface of microcarrier sphere is a network structure, and microcarrier sphere comprises polysaccharide, uronic acid and protein, and then The surface of the microcarrier sphere is coated with a surface treatment layer, and the surface treatment layer includes protein and polylysine. The distance between the cell attachment sites provided by the microcarrier balls is smaller than the cell diameter, that is, the distance between the hydroxyl, carboxyl, and amino sites provided by polysaccharides, proteins, and carboxyl compounds does not exceed 30um. Aldehydic acid in microcarrier spheres can also be effectively replaced by other components rich in hydroxyl, carboxyl, and amino groups.

[0027] The present i...

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Abstract

The invention discloses a composite microcarrier for manufacturing cell culture meat and a preparation method thereof. The composite microcarrier comprises microcarrier balls, the surfaces of the microcarrier balls are of a net-shaped structure, the microcarrier balls comprise polysaccharide, aldehyde acid and protein, the molar percentage of the polysaccharide to the aldehyde acid to the protein is (70-80%): (15-25%): (3-5%), a surface treatment layer is arranged on the surface of each microcarrier ball, the surface treatment layer comprises protein and polylysine, and the molar ratio of the protein to the polylysine is (35-90%): (10-20%). According to the invention, adherent myoblasts can be promoted to rapidly enter a differentiation stage, and then the myoblasts are promoted to realize a myogenic function.

Description

technical field [0001] The invention relates to a composite microcarrier used for cell culture meat production and a preparation method thereof. Background technique [0002] The production of cell-cultured meat has the potential to provide real meat products, and exploring more efficient methods of cell culture and differentiation is an important issue facing the production of cell-cultured meat worldwide. [0003] Cell culture can be used for large-scale cell production through fermenters, cell factories, etc., but there are few reports on cell differentiation and myogenic research. Especially in the research on the differentiation direction of myoblasts, there is no published carrier that can effectively regulate and promote the differentiation of myoblasts. Therefore, related equipment, methods and carriers that can effectively promote myoblast differentiation in cell cultured meat research need to be solved urgently. Contents of the invention [0004] In order to so...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2531/00C12N2533/30C12N2533/70C12N2533/50
Inventor 王守伟杨峰李莹莹李石磊刘文婷李雨爽
Owner CHINA MEAT RES CENT
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