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Grafting method for grafting blueberries by using vaccinium bracteatum as stocks

A black rice tree and rootstock technology, applied in the field of fruit tree grafting, can solve the problems of serious blueberry disease, immature blueberry grafting technology, etc., and achieve the effects of improving taste, cold resistance and disease resistance

Pending Publication Date: 2021-11-30
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a grafting method for grafting blueberries using black rice tree as rootstock, so as to solve the problem that the related grafting technology of blueberries on the market is immature and the blueberry disease is serious

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  • Grafting method for grafting blueberries by using vaccinium bracteatum as stocks
  • Grafting method for grafting blueberries by using vaccinium bracteatum as stocks
  • Grafting method for grafting blueberries by using vaccinium bracteatum as stocks

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Effect test

Embodiment 1

[0040] Embodiment 1: Utilize black rice tree as stock grafting blueberry

[0041] 1 material

[0042] In the present invention, the black rice tree of Siming Mountain grown at an altitude of more than 800 meters is used as the rootstock, and the cultivar O'Neill of the blueberry is used as the scion. Grafting experiment was carried out in Xikou blueberry base in Fenghua.

[0043] 2 methods

[0044] 2.1 Grafting

[0045] Select spring and autumn for grafting, and adopt the branch grafting method to graft the blueberry O'Neill scion to the rootstock of the black rice tree. The specific grafting method is as follows:

[0046] (1) Scion selection

[0047] Select O'Neill blueberry plant and enter the dormant non-flowering bud branch as the scion, and the diameter of the scion requires greater than 5mm and less than 10mm, and then directly grafts;

[0048] (2) Grafting time

[0049] Choose spring February 20-March 10 and autumn October 10-October 30 for grafting, adopt the bra...

Embodiment 2

[0061] Embodiment 2: the gene expression level test of grafting material and female parent:

[0062] In order to explore the differences in gene expression between the grafted material and the maternal material, in this example, the grafted material and the maternal material were subjected to transcriptome sequencing, and differential gene expression analysis was performed. The results showed that the number of differentially expressed genes between the grafted material F1 and the P1 of the female parent of A. spp. 25828vs 25568 and 19657vs21003) (eg figure 1 shown), which indicated that the gene expression patterns were quite different among different species, but the gene expression patterns were very similar among the same species, and grafting could not significantly change the gene expression patterns. But we can also observe that there are 16146 differentially expressed genes between the grafted material F1 and blueberry parent P1 (such as figure 1 Shown in A), which als...

Embodiment 3

[0064] Embodiment 3: resistance gene test in the grafting material:

[0065] Disease experiments showed that the grafted material F1 was more disease-resistant than the blueberry parent P2. There are two kinds of plant resistance responses, one is plant constitutive disease resistance response, and the other is plant induced disease resistance response. The plant's constitutive disease resistance response is that plants will form specific tissues to resist the damage of pests and diseases, such as cutin and wax, which can prevent the damage of pests and diseases. In Arabidopsis, when encountering injury stress, the leaf surface will release cutin and wax to achieve the purpose of repairing the injury. In our transcriptome differentially expressed gene data, we found that the cutin and wax synthesis genes CYP86 and CER1 were up-regulated in the grafted material F1 compared with the blueberry parent P2 (e.g. figure 2 shown in A). In addition, we found that the CYP86 and CER1...

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Abstract

The invention discloses a grafting method for grafting blueberries by using vaccinium bracteatum as stocks, and belongs to the field of fruit tree grafting. The method comprises the following steps: selecting stocks and scions, treating before grafting and grafting. According to the method, the blueberries are grafted by using vaccinium bracteatum as the stocks and the blueberries as scion materials through a designed grafting technology. By designing a proper grafting mode and cultivation means, the disease resistance of the blueberries is effectively improved, the taste of the blueberry fruits is improved, the cold resistance of the blueberry fruits is improved, the scion materials can be promoted to simultaneously obtain the excellent characters of two female parents in the grafting process, and the scion materials can generate the production character with the economic value required by people, so that the development and application of economic plants can be greatly promoted.

Description

technical field [0001] The invention relates to the field of fruit tree grafting, and more specifically relates to a grafting method for grafting blueberries using black rice tree as a stock. Background technique [0002] Black rice tree (Vaccinium bracteatum Thunb), also known as Nanzhu, was called dyed shun in ancient times. It is an evergreen shrub of the Rhododendron family (Ericaceae). Distributed in the southern part of my country, it can be planted in most parts of China. It is born on hillsides, roadsides or bushes. It is an evergreen plant species. The leaves are reddish in autumn and emerald green in summer. It is barren, has strong germination ability, well-developed root system and strong disease resistance. It has a wide range of suitable growth and has new disease resistance genes. [0003] Blueberry (Vaccinium myrtillus), belongs to Rhododendron (Ericaceae) Vaccinium (Vaccinium) Vaccinium plant. Because of its unique health effects, it is known as the "21st ...

Claims

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Application Information

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IPC IPC(8): A01G2/30
CPCA01G2/30
Inventor 杨挺李小辉严成其王芳朱红芬沈岚张国方毕继安钱福康赵欢成
Owner NINGBO ACAD OF AGRI SCI
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