Bacillus subtilis, phaffia rhodozyma and rhizopus mixed tobacco fermentation method

A technology of Bacillus subtilis and Phaffia rhodozyma, applied in the field of microorganisms, can solve the problems of insufficient aroma quantity, high irritation, monotonous aroma quality and the like, and achieve the effects of improving aroma quality and aroma quantity, improving quality and improving sensory quality.

Pending Publication Date: 2021-12-28
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensory quality of the modulated tobacco leaves has been improved, it cannot be directly used to produce cigarettes due to its high irritation, heavy green odor, monotonous aroma, and insufficient aroma.

Method used

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  • Bacillus subtilis, phaffia rhodozyma and rhizopus mixed tobacco fermentation method
  • Bacillus subtilis, phaffia rhodozyma and rhizopus mixed tobacco fermentation method
  • Bacillus subtilis, phaffia rhodozyma and rhizopus mixed tobacco fermentation method

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Embodiment 1

[0020] Example 1 Bacillus subtilis, rhizopus and Phaffia rhodozyme mixed fermentation tobacco leaves

[0021] (1) Strain activation: Bacillus subtilis (DES-59) and Phaffia rhodozyme (Y119) were inoculated on LB agar medium and YPD agar medium respectively for activation by streaking on the plate, cultured at 37°C for 24 hours, Culture at 22°C for 5-7 days;

[0022] (2) Preparation of seed liquid: Inoculate the single colonies of Bacillus subtilis and Phaffia rhodozyme in the plate of step (1) into LB liquid medium and YPD liquid medium, and cultivate them at 37°C for 20h and 22°C for 28- 29h, OD 600 1.6-1.8, 1.8-2.0 respectively;

[0023] (3) Preparation of mixed bacterial liquid: mixing equal volumes of Bacillus subtilis seed liquid and Phaffia rhodozyme seed liquid to obtain mixed bacterial liquid;

[0024] (4) centrifugation: centrifuge the mixed bacterial liquid of step (3) at a rotating speed of 10000rpm for 5-10min, and collect the bacterial cells;

[0025] (5) Washi...

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Abstract

The invention relates to a mixed tobacco fermentation method. The method comprises the following steps of respectively inoculating bacillus subtilis and phaffia rhodozyma on an LB agar culture medium and a YPD agar culture medium, respectively culturing for 24 hours at 37 DEG C and culturing for 5-7 days at 22 DEG C to obtain single colonies of the bacillus subtilis and the phaffia rhodozyma; respectively inoculating the single colonies into an LB liquid culture medium and a YPD liquid culture medium, respectively culturing for 20 hours at 37 DEG C and culturing for 28-29 hours at 22 DEG C to obtain a bacillus subtilis seed solution and a phaffia rhodozyma seed solution, and mixing the bacillus subtilis seed solution and the phaffia rhodozyma seed solution in an isopyknic manner to obtain a mixed bacterium solution; centrifuging the mixed bacterial liquid, collecting thalli and washing; resuspending the washed thalli to obtain a bacterial suspension; and adding the bacterial suspension and rhizopus powder into tobacco leaves, and fermenting at 25 DEG C for 7-10 days. By means of the method, the bacillus subtilis, the phaffia rhodozyma and the rhizopus are compounded and mixed, so that macromolecules such as starch and protein which have negative effects on the quality of the tobacco leaves can be degraded into alcohols, lipids, aldehydes and aroma small molecular substances, and the quality of the tobacco leaves is effectively improved.

Description

technical field [0001] The invention relates to a method for fermenting tobacco mixed with bacillus subtilis, Phaffia rhodozyme and rhizopus, belonging to the technical field of microbes. Background technique [0002] Tobacco leaf fermentation is an important method to improve the quality of tobacco leaves under natural or artificial intervention conditions after tobacco leaves are modulated. Although the sensory quality of the modulated tobacco leaves has been improved, it cannot be directly used to produce cigarettes because of its high irritation, heavy green miscellaneous odor, monotonous aroma, and insufficient aroma. Fermenting tobacco leaves with microorganisms can significantly improve the above defects and improve the quality of cigarettes. At the same time, in order to meet the requirement that heat-not-burn cigarettes can release more flavor substances at low temperatures, it can not only reduce the harm caused by the ignition and combustion of ordinary cigarette...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P1/04A24B15/20C12R1/125C12R1/645C12R1/845
CPCC12P1/02C12P1/04A24B15/20Y02E50/10
Inventor 胡彬彬邹聪明朱明军麦景宁英晋艳蔺忠龙何悦陈颐姜永雷刘志华刘春波王保兴尚善斋
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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