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Preparation process and application of rod-like colloidal gold particles

A technology of colloidal gold particles and a preparation process, applied in the biological field, can solve the problems of affecting the observation of results, large amount of colloidal gold, insufficient liquid power, etc., and achieve the effects of improving the sensitivity of antigen detection, fast fading, and reducing false negatives.

Pending Publication Date: 2022-01-07
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are various types of kits for detecting antigens or antibodies on the market. Colloidal gold immunochromatography antigen products usually increase the sensitivity by increasing the particle size of colloidal gold. The amount of colloidal gold is large and the chromatography speed is slow, which is easy to cause Insufficient liquid power, slow whitening, easy to produce gold spots on the observation window, affecting the observation of the results

Method used

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  • Preparation process and application of rod-like colloidal gold particles
  • Preparation process and application of rod-like colloidal gold particles
  • Preparation process and application of rod-like colloidal gold particles

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A human immunodeficiency virus P24 antigen detection kit, including a detection card, the detection card is provided with a human immunodeficiency virus P24 antigen detection test strip, and the test strip includes a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and A water-absorbing pad, wherein the colloidal gold pad is combined with a colloidal gold marker, and a detection line and a quality control line are provided on the nitrocellulose membrane;

[0022] The preparation method of test strip comprises the following steps:

[0023] 1. Preparation of Colloidal Gold Pads

[0024] The preparation technology of rod-shaped colloidal gold particle comprises the following steps:

[0025] (1) Solution configuration: take HAuCl 4 and trisodium citrate, respectively configured as 0.01wt% HAuCl 4 Aqueous solution and 0.5wt% trisodium citrate aqueous solution;

[0026] (2) Material-liquid mixing: take 100ml of HAuCl in step (1) 4 Aqueous solut...

Embodiment 2

[0037] A human immunodeficiency virus P24 antigen detection kit, the only difference between the kit and the kit in Example 1 is the preparation of rod-shaped colloidal gold particles:

[0038] (1) Solution configuration: take HAuCl 4 and trisodium citrate, respectively configured as 0.01wt% HAuCl 4 Aqueous solution and 0.8wt% trisodium citrate aqueous solution;

[0039] (2) Material-liquid mixing: take 100ml of HAuCl in step (1) 4 Aqueous solution, when heated to 83°C, add 0.13ml of trisodium citrate aqueous solution in step (1) under stirring, continue heating to boiling, then add 0.3ml of trisodium citrate aqueous solution in step (1) under stirring , stirring and mixing to obtain a boiling reaction feed liquid;

[0040] (3) Heat treatment: Take the reaction feed liquid in the boiling state in step (2), heat it with a heating mantle under the voltage condition of 78V for 7 minutes, and then use the electric heating mantle under the voltage condition of 148V to continue h...

Embodiment 3

[0043]A human immunodeficiency virus P24 antigen detection kit, the only difference between the kit and the kit in Example 1 is the preparation of rod-shaped colloidal gold particles:

[0044] (1) Solution configuration: take HAuCl 4 and trisodium citrate, respectively configured as 0.01wt% HAuCl 4 Aqueous solution and 1wt% trisodium citrate aqueous solution;

[0045] (2) Material-liquid mixing: take 100ml of HAuCl in step (1) 4 Aqueous solution, when heated to 85°C, add 0.15ml of trisodium citrate aqueous solution in step (1) under stirring, continue heating to boiling, then add 0.45ml of trisodium citrate aqueous solution in step (1) under stirring , stirring and mixing to obtain a boiling reaction feed liquid;

[0046] (3) Heat treatment: Take the reaction feed liquid in the boiling state in step (2), heat it with a heating mantle under the voltage condition of 80V for 5 minutes, and then use the electric heating mantle under the voltage condition of 150V to continue hea...

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Abstract

The invention relates to the technical field of biology, in particular to a preparation process and application of colloidal gold particles. The preparation process comprises the following steps of: (1) preparing a solution; (2) mixing feed liquid; (3) performing heating treatment; and (4) performing cooling treatment. By adopting the preparation process disclosed by the invention, 80-100nm rod-like colloidal gold particles can be obtained. When the colloidal gold particles are used for labeling, the antigen detection sensitivity can be improved, especially when a weak positive sample is detected, the detection sensitivity is enhanced, and false negative is reduced. Compared with conventional round colloidal gold particles, the colloidal gold particles are small in dosage, fast in color fading and not prone to generating gold spots.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation process and application of colloidal gold particles. Background technique [0002] There are usually two strategies for pathogen detection in the prior art. One is to detect the pathogen itself, and the other is to detect the antibodies produced by the human body to resist the pathogen. Pathogens can be detected immediately after invading the human body and causing diseases, while antibody detection requires human adaptive immunity. Antibodies with corresponding titers can be detected. Relatively speaking, the window period of antigen detection is shorter, which is more suitable for early detection, especially for some serious infectious diseases. [0003] At present, there are various types of kits for detecting antigens or antibodies on the market. Colloidal gold immunochromatography antigen products usually increase the sensitivity by increasing the particle size of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/577G01N33/58G01N33/548G01N33/532B22F1/00B22F9/24B82Y40/00
CPCG01N33/56988G01N33/56983G01N33/56911G01N33/587G01N33/577G01N33/532G01N33/558G01N33/548B22F9/24B82Y40/00G01N2469/10G01N2333/11
Inventor 杨帆杨致亭曲文英杨明霞王彩云
Owner 山东康华生物医疗科技股份有限公司
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