Chicken feather turning character causal mutation site and application thereof in genetic breeding

Abstract

The invention discloses application of a causal mutation site of a chicken feather turning character in genetic breeding, and belongs to the field of molecular genetic breeding. The invention discloses an application of a causal mutation site of a chicken feather turning character in genetic breeding, the causal mutation site of the chicken feather turning character is located in a 5.24-5.28 M region of a chicken No.33 chromosome, deletion mutation g.5285437-5285451delGATGCCGGCAGGACG exists, and the chicken with the deletion mutation is a feather turning black-bone chicken. Based on genome deep re-sequencing, the causal gene and the causal mutation of the causal gene which influence the feather turning of the black-bone chickens can be quickly and accurately positioned by a one-step method, and quick breeding of a feather turning black-bone chicken group can be realized by taking the causal mutation site of the feather turning as a molecular marker.

Description

technical field [0001] The invention relates to the field of molecular genetic breeding, in particular to a causal mutation site for chicken feather turning and its application in genetic breeding. Background technique [0002] Xiushui yellow-feathered black-bone chicken is a newly discovered local chicken group in Xiushui County, Jiangxi Province. It is said that it has been widely raised in the local area during the late Ming and early Qing Dynasties. Xiushui yellow-feather black-bone chicken is a small-sized meat-type yellow-feather black-bone chicken with medicinal functions. It is suitable for wild stocking. It has the characteristics of small size, strong wildness, wide adaptability and resistance to rough feeding. Xiushui yellow-feathered black-bone chicken has both flat-feathered individuals and rolled-feathered (curly-feathered) individuals. [0003] Black-bone chicken with feathers has always been used as a medicinal record in Chinese traditional medicine. As reco...

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Problems solved by technology

The research method is simple and rough, and the gene mutations found in the research results are only related, not the causal mutation sites of feather turning, so the application of molecular breeding for later turning feather cannot achieve accurate breeding effect;

[0006] (2) The existing patent "Interval positioning method and application of the main gene of the chicken feather trait" (patent number: CN106967832 A) by constructing the F2 generation resource population with the trait of curl feather, and then using the simplified genome sequencing method and the whole genome association Analysis to locate the target interval, and finally use the candidate gene method to screen the main effect gene. The implementation of the entire identification plan takes a long time (the construction of the experimental population is generally at least two years), and the technical processing procedures are many and cumbersome. Genomic marker information is incomplete, which can only be used for marker linkage mapping or association mapping analysis, and cannot directly identify causal genes and causal mutation sites, and cannot quickly and accurately locate target genes and mutation sites

[0007] Based on the above content, it can be seen that there is no causal gene and its causal mutation that can quickly and accurately locate the causal gene that affects the feather turning of Xiushui yellow-feathered black-bone chicken in the prior art

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