Microalgae large-scale culture method based on microalgae flash effect

A technology of flash effect and cultivation method, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of affecting the lighting effect, light inhibition, etc., to improve growth rate, save light energy consumption, improve light Effective use of efficiency

Pending Publication Date: 2022-02-08
广西源藻生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the prior art, most of the cultivation methods start research on the influence factors of light cycle and light intensity on the growth of microalgae. If the constant light intensity is adopted, the growth of microalgae may be photoinhibited; The density in the logarithmic phase of growth keeps increasing, and the density reaches the maximum after reaching the stable phase. At this time, the microalgae cells affect the light effect due to mutual shading. How to effectively increase the growth rate of microalgae has become a research direction.

Method used

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  • Microalgae large-scale culture method based on microalgae flash effect
  • Microalgae large-scale culture method based on microalgae flash effect

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Experimental program
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Effect test

Embodiment 1

[0027] Microalgae pre-cultivation: Inoculate a small amount of Microcystis aeruginosa into the Erlenmeyer flask for pre-cultivation, use BG11 medium, the inoculum amount is 1%, use flashing LED lights for culture, control the culture conditions temperature is 24 ℃, light intensity is 2000Lux , keep the intensity of LED lighting constant and cultivate for 3 days, and quickly enter the logarithmic phase of growth;

[0028] After the microalgae entered the logarithmic phase of growth, the temperature of the culture conditions was controlled at 24°C, and the microalgae were cultured with light by using flashing LED lights. The light time ratio was 14:10h, and the stroboscopic frequency was 60 times / minute; cultured for 7 days until the microalgae entered the stable phase, harvested the microalgae, filtered and dried to constant weight, and calculated the biological yield.

Embodiment 2

[0030] Microalgae pre-cultivation: inoculate a small amount of Microcystis aeruginosa into the triangular flask for pre-cultivation, use BG11 medium, the inoculum amount is 0.5%, use flashing LED lights for culture, control the culture conditions temperature is 24 ℃, light intensity is 2000Lux , keep the intensity of the LED light constant and cultivate for 3 days, and quickly enter the logarithmic phase of growth;

[0031] After the microalgae entered the logarithmic phase of growth, the temperature of the culture conditions was controlled at 24°C, and the microalgae were cultured with light by using flashing LED lights. The light time ratio was 14:10h, and the stroboscopic frequency was 60 times / min; cultured for 14 days until the microalgae entered the stable phase, harvested the microalgae, filtered and dried to constant weight, and calculated the biological yield.

Embodiment 3

[0033] Microalgae pre-cultivation: Inoculate a small amount of Chlorella vulgaris in a triangular flask for pre-cultivation, use BG11 medium, the inoculum amount is 0.8%, use flashing LED lights for culture, control the culture conditions at a temperature of 25°C, and a light intensity of 1500Lux , keep the intensity of the LED light constant and cultivate for 3 days, and quickly enter the logarithmic phase of growth;

[0034] After the microalgae entered the logarithmic phase of growth, the temperature of the culture conditions was controlled at 30°C, and the microalgae were cultured with light by using a flashing LED lamp. The light time ratio was 14:10h, and the stroboscopic frequency was 50 times / minute; cultured for 12 days until the microalgae entered the stable phase, harvested the microalgae, filtered and dried to constant weight, and calculated the biological yield.

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Abstract

The invention provides a microalgae large-scale culture method based on a microalgae flash effect. The method comprises the following steps: culturing inoculated microalgae to a growth logarithmic phase by adopting a flash LED lamp; and culturing the microalgae entering the logarithmic growth phase by stroboscopic illumination until the progress is stable. According to the invention, not only are the illumination period and illumination intensity in the microalgae culture process researched, but also the flicker frequency of a light source and the adjustment of the frequent cycle times of microalgae cells between a light area and a dark area are researched, the light energy utilization efficiency of a microalgae light system is effectively promoted, the light inhibition phenomenon of microalgae is reduced while the growth rate of microalgae is improved.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a method for large-scale cultivation of microalgae based on the flash effect of microalgae. Background technique [0002] With the continuous improvement of living standards, the discharge of nutrients in the environment is increasing day by day, and the pollution of water bodies is becoming more and more serious. Seriously endanger the survival of other organisms in the water body, and even human health. The high levels of algal toxins released by algae in the environment have negatively impacted public health, livestock and water supplies. In order to effectively prevent and control microalgal blooms, in-depth research on algae is particularly important. [0003] In order to understand the natural conditions of algae blooms, there are four main methods for the large-scale cultivation of microalgae: open pond culture, closed pond culture, closed ligh...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N13/00C12R1/89
CPCC12N1/12C12N13/00
Inventor 王长海刘瑞卿何梅琳王烨
Owner 广西源藻生物科技有限公司
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