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Tumor infiltrating lymphocyte analysis method and device and storage medium

A lymphocyte and tumor infiltration technology, applied in the field of bioinformatics, can solve problems such as lack and evaluation of TILs subsets, and achieve the effect of improving analysis efficiency and reducing manual calculation and analysis work

Active Publication Date: 2022-02-08
深圳裕策生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since TILs are a group of cells with large heterogeneity, further refinement and quantification of specific indicators are needed, but there is still a lack of effective biomarkers to objectively evaluate TILs subsets

Method used

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  • Tumor infiltrating lymphocyte analysis method and device and storage medium
  • Tumor infiltrating lymphocyte analysis method and device and storage medium
  • Tumor infiltrating lymphocyte analysis method and device and storage medium

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Experimental program
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Embodiment 1

[0117] In this embodiment, 8 cases of surgical tissue slices of patients with clinical lung cancer were used as tumor tissue samples, and the use of Bond RX automated stains to complete multiple immunohistochemical staining on the patient's tumor tissue sample (7 color 6 standard: Foxp3, PD-L1 PANCK, PD-1, CD8, CD68, DAPIs, and full scanning of the Vectra Polaris system to obtain immunohistochemical motors.

[0118] Further, 8 target areas (including tumor tissue, boundary and matrix areas) are selected for each sample of the MIHC pathological image of each case (including tumor tissue, boundary and matrix region) to introduce the INFORM system. figure 2 The target area of ​​the sample 21R2667SLZA is selected (DAPI blue, CD8 cyan, PD-L1 green, foxp3 yellow, PD-1 orange, Pansck red, cd68 white).

[0119] Morphological identification parameter in accordance with the following tissues: Relative intensity threshold parameter is set to 0.1, Nuclear staining quality parameters Mixed, Nu...

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Abstract

The invention discloses a tumor infiltrating lymphocyte analysis method and device and a storage medium, and the method comprises the steps of obtaining a pathological image of a to-be-detected tumor tissue slice, the pathological image being a multiple immunohistochemical staining image or a multiple immunofluorescent staining image; identifying phenotypes of different cells according to the target marker expression signal of the pathological image so as to obtain cell phenotype data corresponding to the target marker; and performing quantitative and / or qualitative analysis on the tumor infiltrating lymphocytes according to the cell phenotype data corresponding to the target marker. According to the tumor infiltrating lymphocyte analysis method, TILs subgroups are deeply analyzed through mIHC / IF detection, specific immune cell types can be analyzed, the relation between immune cells of different parts of a tumor can be integrated, more immunotherapy prognosis data and prediction data are obtained, and the problem that tumor immunotherapy curative effect evaluation is lack of biomarkers is solved.

Description

Technical field [0001] The present application relates to the field of biological information technology, and more particularly to a tumor infiltration lymphocyte analysis method, device, and storage medium. Background technique [0002] Lung cancer is a clinically common malignant tumor, its incidence rate and mortality are the first of malignant tumors, seriously threatening the health and quality of life of patients. Due to the low early diagnostic rate, most patients have been late during diagnosis. The 5-year survival rate of patients currently transferred in low or distantly transferred is only 26% and 4% respectively. In recent years, immune checkpoint inhibitors, ICIS, particularly procedural cell death receptors 1 (Programmed Cell Death Ligand 1, PD-L1) Inhibitors have improved the prognosis of patients with advanced non-small cell lung cancer (NSCLC) patients due to their general admissibility, significant anti-tumor activity, and Non-Smallcell Lung Cancer, NSCLC. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00
CPCG06T7/0012G06T2207/10064G06T2207/10056G06T2207/30024G06T2207/30061G06T2207/30096G06T2207/30242
Inventor 金皓玄李晓婷王佳茜苏小凡
Owner 深圳裕策生物科技有限公司
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