Chloroplast molecular marker primers, and application thereof in early identification of seed sources of tobacco varieties

Abstract

The invention relates to molecular marker primers, and application thereof in early identification of seed sources of tobacco varieties. The molecular marker primers are trnH1 / psbA1; trnH2 / psbA2; trnH3 / psbA3; and trnH4 / psbA4. The molecular marker primers are applied to early identification of seed sources of tobacco varieties. The markers have the beneficial effects that any one pair of the four pairs of primers can be used for distinguishing and identifying conventional tobacco seeds and sterile line seeds in the prior art, that is, molecular marking of the conventional tobacco seeds and the sterile line seeds is completed, so that strict management and control of the tobacco seeds are realized. By using the method disclosed by the invention, distinguishing can be carried out by naked eyes, so that the sterile line tobacco species can be identified.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the field of chloroplast molecular marker primers and application technology for early identification of tobacco variety seed sources. Background technique [0002] The control of tobacco seeds is very strict, and the seeds used in tobacco production include conventional seeds and sterile line seeds. Conventional species can self-pollinate, producing seeds that the next generation can continue to plant. Tobacco male sterile line seeds are cytoplasmic male sterile type. The stamens controlled by cytoplasmic genes are abnormally developed or pollen is aborted, unable to self-pollination, and no seeds are produced. However, when tobacco farmers plant, they may harvest the conventional seeds planted for the next year’s tobacco planting, pretending to be sterile line seeds, and before the tobacco plants bloom, the fertility of the plants cannot be discerned with the naked eye, so , seri...

AI Technical Summary

Problems solved by technology

However, when tobacco farmers plant, they may harvest the conventional seeds planted for the next year’s tobacco planting, pretending to be sterile line seeds, and before the tobacco plants bloom, the fertility of the plants cannot be discerned with the naked eye, so , which seriously affects the double-control policy of tobacco production and poses a challenge to the sustainable development of tobacco production

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