Efficient pig raising method and culture system capable of improving maturation rate of oocytes

Abstract

The invention relates to the field of cell culture, and particularly discloses an efficient pig raising method and culture system capable of improving the maturation rate of oocytes. The system comprises a carbon dioxide incubator and a control panel. A sliding plate is arranged in the carbon dioxide incubator, and a group of culture dishes are arranged in the sliding plate. Liquid supplementing assemblies are arranged inside and outside the carbon dioxide incubator, and a liquid discharging assembly is arranged inside the carbon dioxide incubator. The control panel is mounted on the outer side of the carbon dioxide incubator, and a timer is mounted on the outer side of the carbon dioxide incubator. According to the invention, the replacement of an oocyte culture solution is conveniently and automatically completed, the workload of researchers is reduced, meanwhile, the culture environment of the oocytes cannot be changed, the observation and recording of the culture condition of the oocytes are also very convenient, and the method and the system are convenient to popularize and use.

Description

technical field [0001] The invention relates to the field of cell culture, and specifically discloses a high-efficiency pig raising method and a culture system for improving the maturation rate of oocytes. Background technique [0002] Mammalian oocyte in vitro culture technology (Invitromaturation, IVM) is an important part of embryo engineering. It refers to the immature oocytes taken out of follicles, which are matured and cultured in vitro, and developed to the second meiotic division metaphase, which can be carried out in vitro. The technology of fertilization and splitting into embryos. As a commonly used model organism, pigs have been studying the in vitro maturation of oocytes since the late 1980s. After years of development, although great progress has been made, in vitro Mature porcine oocytes still have a large gap in their mature quality and developmental potential compared with those in vivo. [0003] In the prior art, in the oocyte culture process, once the cu...

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Problems solved by technology

[0002] Mammalian oocyte in vitro culture technology (Invitromaturation, IVM) is an important part of embryo engineering. It refers to the immature oocytes taken out of follicles, which are matured and cultured in vitro, and developed to the second meiotic division metaphase, which can be carried out in vitro. The technology of fertilization and splitting into embryos. As a commonly used model organism, pigs have been studying the in vitro maturation of oocytes since the late 1980s. After years of development, although great progress has been made, in vitro Mature porcine oocytes still have a large gap in their mature quality and developmental potential compared with those in vivo

[0003] In the prior art, in the oocyte culture process, once the culture medium needs to be replaced and the oocyte culture situation needs to be observed, the researchers need to take the oocyte out of the incubator and replace it manually, which is a cumbersome process. Moreover, the culture environment of oocytes changes, which affects the culture effect and is inconvenient to use. Therefore, we have introduced a high-efficiency pig raising method and a culture system to improve the maturation rate of oocytes to solve such problems

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